Differential recognition patterns of Schistosoma haematobium adult worm antigens by the human antibodies IgA, IgE, IgG1 and IgG4

Mutapi, F., Bourke, C., Harcus, Y., Midzi, N., Mduluza, T., Turner, C.M.R., Burchmore, R.J.S. and Maizels, R.M. (2010) Differential recognition patterns of Schistosoma haematobium adult worm antigens by the human antibodies IgA, IgE, IgG1 and IgG4. Parasite Immunology, 33(3), pp. 181-192. (doi:10.1111/j.1365-3024.2010.01270.x)

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Publisher's URL: http://dx.doi.org/10.1111/j.1365-3024.2010.01270.x

Abstract

Abstract Schistosoma haematobium antigen recognition profiles of the human isotypes IgA, IgE, IgG1 and IgG4 were compared by image analysis of western blots. Adult worm antigens separated by 2-dimensional gel electrophoresis were probed with pooled sera from Zimbabweans resident in a S. haematobium endemic area, followed by identification of individual antigenic parasite proteins using mass spectrometry. Overall, IgG1 reacted with the largest number of antigens, followed by IgE and IgA which detected the same number, while IgG4 detected the fewest antigens. IgE recognised all the antigens reactive with IgG4 as well as an additional four antigens; an isoform of 28kDaGST, phosphoglycerate kinase, actin 1 and calreticulin. IgG1 additionally recognised fatty acid binding protein, triose-phosphate isomerase, and heat shock protein 70, which were not recognised by IgA. Recognition patterns varied between some isoforms e.g. the 2 fructose 1-6-bis-phosphate aldolase isoforms differentially recognised by IgA and IgG1. Although the majority of S. haematobium adult worm antigens are recognised by all the four isotypes there are clear restrictions in antibody recognition for some antigens. This may partly explain differences observed in isotype dynamics at a population level. Differential recognition patterns for some isoforms indicated in the study have potential importance for vaccine development

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Turner, Professor Charles and Burchmore, Dr Richard
Authors: Mutapi, F., Bourke, C., Harcus, Y., Midzi, N., Mduluza, T., Turner, C.M.R., Burchmore, R.J.S., and Maizels, R.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Parasite Immunology
Publisher:Wiley-Blackwell Publishing Ltd.
ISSN:0141-9838
ISSN (Online):1365-3024
Copyright Holders:Copyright © 2010 Wiley-Blackwell
First Published:First published in Parasite Immunology 33(3):181-192
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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