Changes of acute phase protein (haptoglobin, fibrinogen and ceruloplasmin) levels in weaning rabbits after experimental infection With E. coli

Mirchev, G.T., Penchev, G.I., Taneva, S., Vachkov, A., Petrov, V., Eckersall, P.D. , Sotirov, L., Lazarov, L., Christova, T.S. and Nikolov, J. (2009) Changes of acute phase protein (haptoglobin, fibrinogen and ceruloplasmin) levels in weaning rabbits after experimental infection With E. coli. Revue de Medecine Veterinaire, 160(3),

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Abstract

Because infection with E. coli commonly cause diarrhea in weaned rabbits, this study was conducted in order to monitor changes in circulating concentrations of haptoglobin (Hp), fibrinogen (Fb) and ceruloplasmin (Cp) during experimental infection weaned rabbits. For this, 18 rabbits from 40 to 45 days, weaned at 4 weeks were used: 12 were inoculated with strain 015: H - E. coli (6.10 7 cfu) and the other 6 were used as negative controls. Blood samples were performed 24 and 72 hours and 7, 11, 18 and 30 days after inoculation in order to determine the concentrations of acute phase proteins (APP) and coliforms were investigated in faecal samples 1, 6, 44, 16, 25 and 31 days later. The excretion of experimental strain began two days after inoculation with 3 rabbits and strengthened in the 3 rd day while mild to severe diarrhea were observed between the 5 th and 21 th days in all rabbits inoculated except 2. In parallel, bacterial excretion gradually decreased. In addition, Hp concentrations were significantly increased at 24 hours after inoculation with E. Coli, reached maximum values on 7 th day (they were multiplied by 9) and remained significantly elevated compared to baseline until 30 th day. By cons, significant variation compared to baseline concentrations and Cp Fb appeared later (respectively the 3 rd and 7 th days), less intense (maximum values obtained on Day 11 was the approximate than doubled) and less durable (normal values were recovered on 30 th and 18 th days, respectively). Increases in all APPs concentrations were associated with intensity of diarrhea. Positive but moderate correlations were obtained between the concentrations of Hp and Cp or Fb whereas Cp concentrations and Fb were strongly correlated. The evolution in time and amplitude changes in levels of these APPs lead to consider the Cp and Fb proteins as positive but slow evolution of the acute phase while 1'Hp can be classified as a fast protein major and allowing early detection of bacterial infections in weaned rabbits before the onset of clinical signs (diarrhea) and fecal coliform excretion.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor David
Authors: Mirchev, G.T., Penchev, G.I., Taneva, S., Vachkov, A., Petrov, V., Eckersall, P.D., Sotirov, L., Lazarov, L., Christova, T.S., and Nikolov, J.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Revue de Medecine Veterinaire
ISSN:0035-1555

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