UV-B Action spectrum for UVR8-mediated HY5 transcript accumulation in Arabidopsis

Brown, B., Headland, L.R. and Jenkins, G. (2009) UV-B Action spectrum for UVR8-mediated HY5 transcript accumulation in Arabidopsis. Photochemistry and Photobiology, 85(5), pp. 1147-1155. (doi:10.1111/j.1751-1097.2009.00579.x)

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Abstract

<i>Arabidopsis thaliana</i>UV RESISTANCE LOCUS8 (UVR8) is a UV-B-specific signaling component that mediates low fluence, photomorphogenic responses to UV-B. It is required for UV-B-induced expression of the gene encoding the ELONGATED HYPOCOTYL5 (HY5) transcription factor. HY5 is a key effector of responses mediated by UVR8. In mature leaf tissue, HY5 transcript accumulation occurred rapidly in response to a brief UV-B treatment and no induction was observed in a uvr8 mutant over a broad range of UV wavelengths. In response to monochromatic light, maximal transcript accumulation occurred in wild-type plants at wavelengths 280–300 nm. HY5 transcript accumulation showed reciprocity between the fluence rate and duration of UV-B exposure, and on this basis conditions were chosen to generate an action spectrum for the UVR8 signaling pathway. Dose–response curves were produced for a range of UV wavelengths using 20 min exposure to UV and harvesting tissue 2 h after the start of illumination. Experiments using mutants defective in sinapate ester and flavonoid biosynthesis indicated that the presence of UV-absorbing compounds did not affect the construction of an action spectrum under the conditions employed. The action spectrum for the induction of HY5 by the UVR8 pathway showed a main peak at 280 nm with a smaller peak at 300 nm. The data are discussed in relation to the proposed mechanisms of UV-B photoreception.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Brown, Dr Bobby and Jenkins, Professor Gareth
Authors: Brown, B., Headland, L.R., and Jenkins, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Photochemistry and Photobiology
Journal Abbr.:Photochem. Photobiol.
Publisher:Wiley
ISSN:0031-8655
Published Online:22 June 2009

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
419341Characterisation of a UVB specific signalling pathwayGareth JenkinsBiotechnology and Biological Sciences Research Council (BBSRC)BB/D019281/1Institute of Molecular Cell and Systems Biology