Authenticating production origin of Gilthead Sea Bream (Sparus aurata) by chemical and isotopic fingerprinting

Morrison, D. , Preston, T. , Bron, J.E., Hemderson, R.J., Cooper, K., Strachan, F. and Bell, J.G. (2007) Authenticating production origin of Gilthead Sea Bream (Sparus aurata) by chemical and isotopic fingerprinting. Lipids, 42(6), pp. 537-545. (doi:10.1007/s11745-007-3055-3)

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Abstract

Recent EU legislation (EC/2065/2001) requires that fish products, of wild and farmed origin, must provide consumer information that describes geographical origin and production method. The aim of the present study was to establish methods that could reliably differentiate between wild and farmed European gilthead sea bream (Sparus aurata). The methods that were chosen were based on chemical and stable isotopic analysis of the readily accessible lipid fraction. This study examined fatty acid profiles by capillary gas chromatography and the isotopic composition of fish oil (δ<sup>13</sup>C, δ<sup>18</sup>O), phospholipid choline nitrogen (δ<sup>15</sup>N) and compound specific analysis of fatty acids (δ<sup>13</sup>C) by isotope ratio mass spectroscopy as parameters that could reliably discriminate samples of wild and farmed sea bream. The sample set comprised of 15 farmed and 15 wild gilthead sea bream (Sparus aurata), obtained from Greece and Spain, respectively. Discrimination was achieved using fatty acid compositions, with linoleic acid (18:2n-6), arachidonic acid (20:4n-6), stearic acid (18:0), vaccenic acid (18:1n-7) and docosapentaenoic acid (22:5n-3) providing the highest contributions for discrimination. Principle components analysis of the data set highlighted good discrimination between wild and farmed fish. Factor 1 and 2 accounted for >70% of the variation in the data. The variables contributing to this discrimination were: the fatty acids 14:0, 16:0, 18:0, 18:1n-9, 18:1n-7, 22:1n-11, 18:2n-6 and 22:5n-3; δ<sup>13</sup>C of the fatty acids 16:0, 18:0, 16:1n-7, 18:1n-9, 20:5n-3 and 22:6n-3; Bulk oil fraction δ<sup>13</sup>C; glycerol/choline fraction bulk δ<sup>13</sup>C; δ<sup>15</sup>N; % N; % lipid.

Item Type:Articles
Keywords:Sea bream; product authentication; fatty acid compositions; isotope ratio mass spectrometry (IRMS); flesh oil delta 13C; flesh oil delta 18O; glycerol choline fraction delta 15N; Principal components analysis
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Preston, Professor Thomas and Morrison, Dr Douglas
Authors: Morrison, D., Preston, T., Bron, J.E., Hemderson, R.J., Cooper, K., Strachan, F., and Bell, J.G.
Subjects:G Geography. Anthropology. Recreation > GE Environmental Sciences
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Lipids
Publisher:Springer

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