Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy

Millington, O. R., Brewer, J. M. , Garside, P. and Maffia, P. (2010) Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy. In: Marelli-Berg, F. M. and Nourshargh, S. (eds.) T-Cell Trafficking: Methods and Protocols. Series: Methods in Molecular Biology, 616 (616). Humana Press Inc, pp. 193-206. ISBN 9781607614609 (doi:10.1007/978-1-60761-461-6_13)

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Several recent studies in immunology have used multiphoton laser-scanning microscopy to visualise the induction of an immune response in real time in vivo. These experiments are illuminating the cellular and molecular interactions involved in the induction, maintenance and regulation of immune responses. Similar approaches are being applied in cardiovascular research where there is an increasing body of evidence to support a significant role for the adaptive immune system in vascular disease. As such, we have begun to dissect the role of T lymphocytes in atherosclerosis in real time in vivo. Here, we provide step-by-step guides to the various stages involved in visualising the migration of T cells within a lymph node and their infiltration into inflamed tissues such as atherosclerotic arteries. These methods provide an insight into the mechanisms involved in the activation and function of immune cells in vivo.

Item Type:Book Sections
Keywords:Brachiocephalic artery, imaging, lymph node, multiphoton microscopy, T cells
Glasgow Author(s) Enlighten ID:Garside, Professor Paul and Brewer, Professor James and Maffia, Professor Pasquale and Millington, Dr Owain
Authors: Millington, O. R., Brewer, J. M., Garside, P., and Maffia, P.
Subjects:R Medicine > RZ Other systems of medicine
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Publisher:Humana Press Inc
Copyright Holders:Copyright © 2010 Humana Press
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
404921Investigating the role of T cells in vascular pathologyPaul GarsideBritish Heart Foundation (BHF)PG/6/083/211998Infection Immunity and Inflammation Medicine