Advances in imaging of new targets for pharmacological intervention in stroke: real-time tracking of T-cells in the ischaemic brain

Ortolano, F., Maffia, P. , Dever, G., Millington, O., De Simoni, M., Brewer, J. , Bushell, T.J., Garside, P. and Carswell, H.V. (2010) Advances in imaging of new targets for pharmacological intervention in stroke: real-time tracking of T-cells in the ischaemic brain. British Journal of Pharmacology, 159(4), pp. 808-811. (doi:10.1111/j.1476-5381.2009.00527.x)

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Publisher's URL: http://dx.doi.org/10.1111/j.1476-5381.2009.00527.x

Abstract

<p><b>Background and purpose:</b> T-cells may play a role in the evolution of ischaemic damage and repair, but the ability to image these cells in the living brain after a stroke has been limited. We aim to extend the technique of real-time <i>in situ</i> brain imaging of T-cells, previously shown in models of immunological diseases, to models of experimental stroke.</p> <p><b>Experimental approach:</b> Male C57BL6 mice (6–8 weeks) (n = 3) received a total of 2–5 x 10<sup>6</sup> carboxyfluorescein diacetate succinimidyl ester (CFSE)-labelled lymphocytes from donor C57BL6 mice via i.v. injection by adoptive transfer. Twenty-four hours later, recipient mice underwent permanent left distal middle cerebral artery occlusion (MCAO) by electrocoagulation or by sham surgery under isoflurane anaesthesia. Female hCD2-green fluorescent protein (GFP) transgenic mice that exhibit GFP-labelled T-cells underwent MCAO. At 24 or 48 h post-MCAO, a sagittal brain slice (1500 μm thick) containing cortical branches of the occluded middle cerebral artery (MCA) was dissected and used for multiphoton laser scanning microscopy (MPLSM).</p> <p><b>Key results:</b> Our results provide direct observations for the first time of dynamic T-cell behaviour in living brain tissue in real time and herein proved the feasibility of MPLSM for ex vivo live imaging of immune response after experimental stroke.</p> <p><b>Conclusions and Implications:</b> It is hoped that these advances in the imaging of immune cells will provide information that can be harnessed to a therapeutic advantage.</p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Maffia, Dr Pasquale and Millington, Dr Owain and Garside, Professor Paul and Brewer, Professor James
Authors: Ortolano, F., Maffia, P., Dever, G., Millington, O., De Simoni, M., Brewer, J., Bushell, T.J., Garside, P., and Carswell, H.V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:British Journal of Pharmacology
Journal Abbr.:BJP
ISSN:0007-1188
ISSN (Online):1476-5381
Published Online:10 December 2009

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
404921Investigating the role of T cells in vascular pathologyPaul GarsideBritish Heart Foundation (BHF)PG/6/083/211998Infection Immunity and Inflammation Medicine