Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes

Mihm Carmichael, M., Baker, P.J., Fleming, L.M., Monteiro, A.M. and O'Shaughnessy, P.J. (2008) Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes. Reproduction, 135(2), pp. 253-265. (doi: 10.1530/REP-06-0193) (PMID:18239053)

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Abstract

This study was designed to identify genes that regulate the transition from FSH- to LH-dependent development in the bovine dominant follicle (DF). Serial analysis of gene expression (SAGE) was used to compare the transcriptome of granulosa cells isolated from the most oestrogenic growing cohort follicle (COH), the newly selected DF and its largest subordinate follicle (SF) which is destined for atresia. Follicle diameter, follicular fluid oestradiol (E) and E:progesterone ratio confirmed follicle identity. Results show that there are 93 transcript species differentially expressed in DF granulosa cells, but only 8 of these encode proteins known to be involved in DF development. Most characterised transcripts upregulated in the DF are from tissue development genes that regulate cell differentiation, proliferation, apoptosis, signalling and tissue remodelling. Semiquantitative real-time PCR analysis confirmed seven genes with upregulated (P≤0.05) mRNA expression in DF compared with both COH and SF granulosa cells. Thus, the new genes identified by SAGE and real-time PCR, which show enhanced mRNA expression in the DF, may regulate proliferation (cyclin D2; CCND2), prevention of apoptosis or DNA damage (growth arrest and DNA damage-inducible, β; GADD45B), RNA synthesis (splicing factor, arginine/serine rich 9; SFRS9) and unknown processes associated with enhanced steroidogenesis (ovary-specific acidic protein; DQ004742 [GenBank] ) in granulosa cells of DF at the onset of LH-dependent development. Further studies are required to show whether the expression of identified genes is dysregulated when abnormalities occur during DF selection or subsequent development.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Fleming, Mrs Lynne and Baker, Mr Paul and Monteiro, Dr Ana and O'Shaughnessy, Professor Peter and Mihm Carmichael, Dr Monika
Authors: Mihm Carmichael, M., Baker, P.J., Fleming, L.M., Monteiro, A.M., and O'Shaughnessy, P.J.
Subjects:Q Science > QP Physiology
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Reproduction
Publisher:Society for Reproduction and Fertility
ISSN:1470-1626
ISSN (Online):1741-7899

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
318681Changes in follicular granulosacell gene expression determined by SAGEduring dominant follicle selection in cattleMonika Mihm CarmichaelBiotechnology and Biological Sciences Research Council (BBSRC)17/S17355Large Animal Clinical Sciences and Public Health