D'Emmanuele di Villa Bianca, R., Sorrentino, R., Maffia, P. , Mirone, F., Imbimbo, C., Fusco, F., De Palma, R., Ignarro, L.J. and Cirino, G. (2009) Hydrogen sulfide as a mediator of human corpus cavernosum smooth-muscle relaxation. Proceedings of the National Academy of Sciences of the United States of America, 106(11), pp. 4513-4518. (doi: 10.1073/pnas.0807974105)
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Abstract
Hydrogen sulfide (H2S) is synthesized by 2 enzymes, cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE). L-Cysteine (L-Cys)acts as a natural substrate for the synthesis of H2S. Human penile tissue possesses both CBS and CSE, and tissue homogenates efficiently convert L-Cys to H2S. CBS and CSE are localized in the muscular trabeculae and the smooth-muscle component of the penile artery, whereas CSE but not CBS is also expressed in peripheral nerves. Exogenous H2S [sodium hydrogen sulfide (NaHS)] or L-Cys causes a concentration-dependent relaxation of strips of human corpus cavernosum. L-Cys relaxation is inhibited by the CBS inhibitor, aminoxyacetic acid (AOAA). Electrical field stimulation of human penile tissue, under resting conditions, causes an increase in tension that is significantly potentiated by either propargylglycine (PAG; CSE inhibitor) or AOAA. In rats, NaHS and L-Cys promote penile erection, and the response to L-Cys is blocked by PAG. Our data demonstrate that the L-Cys/H2S pathway mediates human corpus cavernosum smooth-muscle relaxation.
| Item Type: | Articles |
|---|---|
| Keywords: | cystathionine beta-synthase (CBS); cystathionine gamma-lyase (CSE); H2S; penile erection; L-cysteine |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Maffia, Professor Pasquale |
| Authors: | D'Emmanuele di Villa Bianca, R., Sorrentino, R., Maffia, P., Mirone, F., Imbimbo, C., Fusco, F., De Palma, R., Ignarro, L.J., and Cirino, G. |
| Subjects: | Q Science > QP Physiology R Medicine > RS Pharmacy and materia medica |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Proceedings of the National Academy of Sciences of the United States of America |
| Publisher: | National Academy of Sciences |
| ISSN: | 0027-8424 |
| ISSN (Online): | 1091-6490 |
| Published Online: | 02 March 2009 |
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