Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing

Love, A.J., Laird, J., Holt, J., Hamilton, A.J., Sadanandom, A. and Milner, J.J. (2007) Cauliflower mosaic virus protein P6 is a suppressor of RNA silencing. Journal of General Virology, 88(12), pp. 3439-3444. (doi: 10.1099/vir.0.83090-0) (PMID:18024914)

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Abstract

We infected a transgenic Arabidopsis line (GxA), containing an amplicon-silenced 35S : : GFP transgene, with cauliflower mosaic virus (CaMV), a plant pararetrovirus with a DNA genome. Systemically infected leaves showed strong GFP fluorescence and amplicon transcripts were detectable in Northern blots, indicating that silencing of GFP had been suppressed during CaMV-infection. Transgenic Arabidopsis lines expressing CaMV protein P6, the major genetic determinant of symptom severity, were crossed with GxA. Progeny showed strong GFP fluorescence throughout and amplicon transcripts were detectable in Northern blots, indicating that P6 was suppressing local and systemic silencing. However, levels of 21 nt siRNAs derived from the GFP transgene were not reduced. In CaMV-infected plants, the P6 transgene did not reduce levels of CaMV leader-derived 21 and 24 nt siRNAs relative to levels of CaMV 35S RNA. These results demonstrate that CaMV can efficiently suppress silencing of a GFP transgene, and that P6 acts as a silencing suppressor.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sadanandom, Dr Ari and Milner, Dr Joel and Laird, Ms Janet
Authors: Love, A.J., Laird, J., Holt, J., Hamilton, A.J., Sadanandom, A., and Milner, J.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
419821Defence and defence suppression in plant pathogen interactions - the role of CaMV protein P6Joel MilnerBiotechnology and Biological Sciences Research Council (BBSRC)BB/D017319/1Life Sciences Biomolecular Science