Production of C-13 labelled pea flour for use in human digestion and fermentation studies

Edwards, C.A., Zavoshy, R., Khanna, S., Slater, C., Morrison, D.J., Preston, T. and Weaver, L.T. (2002) Production of C-13 labelled pea flour for use in human digestion and fermentation studies. Isotopes in Environmental and Health Studies, 38(3), pp. 139-147. (doi:10.1080/10256010215130)

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Publisher's URL: http://taylorandfrancis.metapress.com/openurl.asp?genre=article&id=doi:10.1080/10256010215130

Abstract

Stable isotope breath tests offer a new approach to the study of digestion and fermentation of carbohydrates in man. In this study, C-13 labelled peas were grown by pulsing 250 ml (CO2)-C- 13 into a sealed growth chamber. A second pulse was added to a portion of the peas to increase the C-13 enrichment. This generated pea flour with an enrichment of 2.36 at.% excess (range 2.09-2.71 n =3) and 8.64 atom % excess (range 7.37-9.78 n =3) respectively. This represented incorporation of an absolute yield of 3.8% of the (CO2)-C-13 into peas in the 'once-labelled' treatment and 7.5% in the 'twice-labelled' treatment. Ingestion of a mixture of the labelled pea flour (300 mg) by two volunteers generated measurable (CO2)-C-13 excretion for breath test analysis. The profile of breath (CO2)-C-13 enrichment increased to a maximum within three hours after consuming the pea flour followed by a decrease almost back to'baseline by 13 hours. Breath (CO2)-C-13 appeared to rise again after this apparent nadir at 13 hours until the end of the sampling period. Mathematical analysis of the data suggested that two peaks best described the profile of breath (CO2)-C-13 up to 13 hours. A third peak was necessary to describe the late rise in breath (CO2)-C-13 enrichment. This use of C-13 enriched pea flour may provide a useful non invasive method for measurement of digestion and fermentation in vivo.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Preston, Professor Thomas and Morrison, Dr Douglas
Authors: Edwards, C.A., Zavoshy, R., Khanna, S., Slater, C., Morrison, D.J., Preston, T., and Weaver, L.T.
Subjects:R Medicine > RC Internal medicine
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Isotopes in Environmental and Health Studies
ISSN:1025-6016

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