Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p

Papadopoulou, K. et al. (2010) Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p. Journal of Cell Science, 123(24), pp. 4374-4381. (doi: 10.1242/jcs.073056)

Abstract

Regulated gene expression makes an important contribution to cell cycle control mechanisms. In fission yeast, a group of genes is coordinately expressed during a late stage of the cell cycle (M phase and cytokinesis) that is controlled by common cis-acting promoter motifs named pombe cell cycle boxes (PCBs), which are bound by a trans-acting transcription factor complex, PCB binding factor (PBF). PBF contains at least three transcription factors, a MADS box protein Mbx1p and two forkhead transcription factors, Sep1p and Fkh2p. Here we show that the fission yeast Cdc14p-like phosphatase Clp1p (Flp1p) controls M–G1 specific gene expression through PBF. Clp1p binds in vivo both to Mbx1p, a MADS box-like transcription factor, and to the promoters of genes transcribed at this cell cycle time. Because Clp1p dephosphorylates Mbx1p in vitro, and is required for Mbx1p cell cycle-specific dephosphorylation in vivo, our observations suggest that Clp1p controls cell cycle-specific gene expression through binding to and dephosphorylating Mbx1p.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McInerny, Dr Chris
• Authors: Papadopoulou, K., Chen, J.-S., Mead, E., Feoktistova, A., Petit, C., Argarwall, M., Jamal, M., Malik, A., Spanos, A., Sedgwick, S.G., Karagiannis, J., Balasubramanian, M.K., Gould, K.L., and McInerny, C.J.
• College/School: College of Medical Veterinary and Life Sciences > School of Life Sciences; College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Journal of Cell Science
• Journal Abbr.: J. Cell Sci.
• ISSN: 0021-9533
• ISSN (Online): 1477-9137
• Published Online: 23 November 2010