Csaderova, L., Martines, E., Seunarine, K., Gadegaard, N. , Wilkinson, C.D.W. and Riehle, M.O. (2010) A biodegradable and biocompatible regular nanopattern for large-scale selective cell growth. Small, 6(23), pp. 2755-2761. (doi: 10.1002/smll.201000193)
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Abstract
A biodegradable substrate with a regular array of nanopillars fabricated by electron-beam lithography and hot embossing is used to address the mechanisms of nanotopographical control of cell behaviour. Two different cell lines cultured on the nanopillars show striking changes in cell coverage. These changes are topography- and cell-dependent, and do not depend on the trapping of air bubbles by the nanopattern. For the first time a strong cell-selective effect of the same nanotopography has been clearly demonstrated on a large area, as fibroblast proliferation is inhibited while endothelial cell spreading is visibly enhanced. Fibroblast proliferation results indicate that a reduction of available surface area induced by nanotopography might be the main factor affecting cell growth on nanopatterns. The results presented herein pave the way towards the development of permanent vascular replacements, where non-adhesive, inert, surfaces will induce rapid in situ endothelialisation to reduce thrombosis and occlusion.
Item Type: | Articles |
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Additional Information: | We would like to thank M Mullen for help with the SEM preparation, Prof. A.S.G. Curtis, A. Hart, A. Macintosh, and G. Aitchison at Glasgow University, and M Preston of Media Cybernetics for their help and discussion. The partial support by the EC-funded project NaPa (Contract no. NMP4-CT-2003-500120), and the EPSRC (grant GR/S13415/01) is gratefully acknowledged. The content of this work is the sole responsibility of the authors. |
Keywords: | Nanotopography, surface, cell adhesion, implant, cell-selective |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Seunarine, Dr Krishna and Riehle, Dr Mathis and Wilkinson, Professor Christopher and Gadegaard, Professor Nikolaj |
Authors: | Csaderova, L., Martines, E., Seunarine, K., Gadegaard, N., Wilkinson, C.D.W., and Riehle, M.O. |
Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
Research Group: | Centre for Cell Engineering |
Journal Name: | Small |
ISSN: | 1613-6810 |
ISSN (Online): | 1613-6829 |
Published Online: | 11 November 2010 |
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