Comparison of the fluorescence kinetics of detergent-solubilized and membrane-reconstituted LH2 complexes from Rps. acidophila and Rb. sphaeroides

Pflock, T., Dezi, M., Venturoli, G., Cogdell, R.J. , Köhler, J. and Oellerich, S. (2008) Comparison of the fluorescence kinetics of detergent-solubilized and membrane-reconstituted LH2 complexes from Rps. acidophila and Rb. sphaeroides. Photosynthesis Research, 95(2-3), pp. 291-298. (doi:10.1007/s11120-007-9245-2)

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Publisher's URL: http://dx.doi.org/10.1007/s11120-007-9245-2

Abstract

Picosecond time-resolved fluorescence spectroscopy has been used in order to compare the fluorescence kinetics of detergent-solubilized and membrane-reconstituted light-harvesting 2 (LH2) complexes from the purple bacteria <i>Rhodopseudomonas</i> (<i>Rps.</i>) <i>acidophila</i> and <i>Rhodobacter</i> (<i>Rb.</i>) <i>sphaeroides</i>. LH2 complexes were reconstituted in phospholipid model membranes at different lipid:protein-ratios and all samples were studied exciting with a wide range of excitation densities. While the detergent-solubilized LH2 complexes from <i>Rps. acidophila</i> showed monoexponential decay kinetics (tf = 980 ps) for excitation densities of up to 3ú1013 photons/(pulseúcm2), the membrane-reconstituted LH2 complexes showed multiexponential kinetics even at low excitation densities and high lipid:protein-ratios. The latter finding indicates an efficient clustering of LH2 complexes in the phospholipid membranes. Similar results were obtained for the LH2 complexes from <i>Rb. sphaeroides</i>.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard
Authors: Pflock, T., Dezi, M., Venturoli, G., Cogdell, R.J., Köhler, J., and Oellerich, S.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Photosynthesis Research
ISSN:0166-8595
Published Online:03 October 2007

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
303032The structure and function of purple bacterial antenna complexesRichard CogdellBiotechnology and Biological Sciences Research Council (BBSRC)BB/D000610/1Institute of Molecular Cell and Systems Biology