A cathepsin L-like protease from Strongylus vulgaris: an orthologue of Caenorhabditis elegans CPL-1

Nic An Ultaigh, S., Carolan, J. C., Britton, C. , Murray, L. and Ryan, M. F. (2009) A cathepsin L-like protease from Strongylus vulgaris: an orthologue of Caenorhabditis elegans CPL-1. Experimental Parasitology, 121(4), pp. 293-299. (doi: 10.1016/j.exppara.2008.11.002) (PMID:19059238)

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Abstract

Cathespin L-like proteases (CPLs), characterized from a wide range of helminths, are significant in helminth biology. For example, in Caenorhabditis elegans CPL is essential for embryogenesis. Here, we report a cathepsin L-like gene from three species of strongyles that parasitize the horse, and describe the isolation of a cpl gene (Sv-cpl-1) from Strongylus vulgaris, the first such from equine strongyles. It encodes a protein of 354 amino acids with high similarity to other parasitic Strongylida (9091%), and C. elegans CPL-1 (87%), a member of the same Clade. As S. vulgaris cpl-1 rescued the embryonic lethal phenotype of the C. elegans cpl-1 mutant, these genes may be orthologues, sharing the same function in each species. Targeting Sv-CPL-1 might enable novel control strategies by decreasing parasite development and transmission.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Murray, Ms Linda and Britton, Professor Collette
Authors: Nic An Ultaigh, S., Carolan, J. C., Britton, C., Murray, L., and Ryan, M. F.
Subjects:Q Science > Q Science (General)
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Experimental Parasitology
Publisher:Elsevier
ISSN:0014-4894
ISSN (Online):1090-2449
Published Online:18 November 2008

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
351421Adaptation of caenorhabditis elegans technology to identification and expression of parasite target genesCollette BrittonBiotechnology and Biological Sciences Research Council (BBSRC)S20387III - PARASITOLOGY