Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein

Owsianka, A.M., Tarr, A.W., Keck, Z.Y., Li, T.K., Witteveldt, J., Adair, R., Foung, S.K.H., Ball, J.K. and Patel, A.H. (2008) Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein. Journal of General Virology, 89(3), pp. 653-659. (doi:10.1099/vir.0.83386-0) (PMID:18272755) (PMCID:PMC2885755)

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Abstract

The humoral response to hepatitis C virus (HCV) may contribute to controlling infection. We previously isolated human monoclonal antibodies to conformational epitopes on the HCV E2 glycoprotein. Here, we report on their ability to inhibit infection by retroviral pseudoparticles incorporating a panel of full-length E1E2 clones representing the full spectrum of genotypes 16. We identified one antibody, CBH-5, that was capable of neutralizing every genotype tested. It also potently inhibited chimeric cell culture-infectious HCV, which had genotype 2b envelope proteins in a genotype 2a (JFH-1) background. Analysis using a panel of alanine-substitution mutants of HCV E2 revealed that the epitope of CBH-5 includes amino acid residues that are required for binding of E2 to CD81, a cellular receptor essential for virus entry. This suggests that CBH-5 inhibits HCV infection by competing directly with CD81 for a binding site on E2.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Owsianka, Dr Anna and Witteveldt, Dr Jeroen and Patel, Professor Arvind
Authors: Owsianka, A.M., Tarr, A.W., Keck, Z.Y., Li, T.K., Witteveldt, J., Adair, R., Foung, S.K.H., Ball, J.K., and Patel, A.H.
Subjects:Q Science > QR Microbiology > QR180 Immunology
Q Science > QR Microbiology > QR355 Virology
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences
Journal Name:Journal of General Virology
Publisher:Society for General Microbiology
ISSN:0022-1317
ISSN (Online):1465-2099

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