Mouw, K.W., Rowland, S.-J., Gajjar, M.M., Boocock, M.R., Stark, W.M. and Rice, P.A. (2008) Architecture of a serine recombinase-DNA regulatory complex. Molecular Cell, 30(2), pp. 145-155. (doi: 10.1016/j.molcel.2008.02.023)
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Abstract
An essential feature of many site-specific recombination systems is their ability to regulate the direction and topology of recombination. Resolvases from the serine recombinase family assemble an inter-wound synaptic complex that harnesses negative supercoiling to drive the forward reaction and promote recombination between properly oriented sites. To better understand the interplay of catalytic and regulatory functions within these synaptic complexes, we have solved the structure of the regulatory site synapse in the Sin resolvase system. It reveals an unexpected synaptic interface between helix-turnhelix DNA-binding domains that is also highlighted in a screen for synapsis mutants. The tetramer defined by this interface provides the foundation for a robust model of the synaptic complex, assembled entirely from available crystal structures, that gives insight into how the catalytic activity of Sin and other serine recombinases may be regulated
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Boocock, Dr Martin and Rowland, Dr Sally-Jane and Stark, Professor Marshall |
| Authors: | Mouw, K.W., Rowland, S.-J., Gajjar, M.M., Boocock, M.R., Stark, W.M., and Rice, P.A. |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH345 Biochemistry |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Molecular Cell |
| ISSN: | 1097-2765 |
| ISSN (Online): | 1097-4164 |
| Published Online: | 24 April 2008 |
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