Architecture of a serine recombinase-DNA regulatory complex

Mouw, K.W., Rowland, S.-J., Gajjar, M.M., Boocock, M.R., Stark, W.M. and Rice, P.A. (2008) Architecture of a serine recombinase-DNA regulatory complex. Molecular Cell, 30(2), pp. 145-155. (doi: 10.1016/j.molcel.2008.02.023)

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An essential feature of many site-specific recombination systems is their ability to regulate the direction and topology of recombination. Resolvases from the serine recombinase family assemble an inter-wound synaptic complex that harnesses negative supercoiling to drive the forward reaction and promote recombination between properly oriented sites. To better understand the interplay of catalytic and regulatory functions within these synaptic complexes, we have solved the structure of the regulatory site synapse in the Sin resolvase system. It reveals an unexpected synaptic interface between helix-turnhelix DNA-binding domains that is also highlighted in a screen for synapsis mutants. The tetramer defined by this interface provides the foundation for a robust model of the synaptic complex, assembled entirely from available crystal structures, that gives insight into how the catalytic activity of Sin and other serine recombinases may be regulated

Item Type:Articles
Glasgow Author(s) Enlighten ID:Boocock, Dr Martin and Rowland, Dr Sally-Jane and Stark, Professor Marshall
Authors: Mouw, K.W., Rowland, S.-J., Gajjar, M.M., Boocock, M.R., Stark, W.M., and Rice, P.A.
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Molecular Cell
ISSN (Online):1097-4164
Published Online:24 April 2008

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