Urinary proteomic diagnosis of coronary artery disease: identification and clinical validation in 623 individuals

Delles, C. et al. (2010) Urinary proteomic diagnosis of coronary artery disease: identification and clinical validation in 623 individuals. Journal of Hypertension, 28(11), pp. 2316-2322. (doi:10.1097/HJH.0b013e32833d81b7)

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Publisher's URL: http://dx.doi.org/10.1097/HJH.0b013e32833d81b7

Abstract

Objectives We studied the urinary proteome in a total of 623 individuals with and without coronary artery disease (CAD) in order to characterize multiple biomarkers that enable prediction of the presence of CAD. Methods Urine samples were analyzed by capillary electrophoresis coupled online to micro time-of-flight mass spectrometry. Results We defined a pattern of 238 CAD-specific polypeptides from comparison of 586 spot urine samples from 408 individuals. This pattern identified patients with CAD in a blinded cohort of 138 urine samples (71 patients with CAD and 67 healthy individuals) with high sensitivity and specificity (area under the receiver operator characteristic curve 87%, 95% confidence interval 81-92) and was superior to previously developed 15-marker (area under the receiver operator characteristic curve 68%, P < 0.0001) and 17-marker panels (area under the receiver operator characteristic curve 77%, P < 0.0001). The sequences of the discriminatory polypeptides include fragments of alpha-1-antitrypsin, collagen types 1 and 3, granin-like neuroendocrine peptide precursor, membrane-associated progesterone receptor component 1, sodium/potassium-transporting ATPase gamma chain and fibrinogen-alpha chain. Several biomarkers changed significantly toward the healthy signature following 2-year treatment with irbesartan, whereas short-term treatment with irbesartan did not significantly affect the polypeptide pattern. Conclusion Urinary proteomics identifies CAD with high confidence and might also be useful for monitoring the effects of therapeutic interventions.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dominiczak, Professor Anna and Delles, Professor Christian and Mischak, Professor Harald and Dymott, Dr Jane and Zimmerli, Dr Lukas and Neisius, Dr Ulf
Authors: Delles, C., Schiffer, E., von zur Muhlen, C., Peter, K., Rossing, P., Parving, H.-H., Dymott, J.A., Neisius, U., Zimmerli, L.U., Snell-Bergeon, J.K., Maahs, D.M., Schmieder, R.E., Mischak, H., and Dominiczak, A.F.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of Hypertension
ISSN:0263-6352

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