MacDonald, A.I., Lu, Y., Kilbride, E.A., Akopian, A. and Colloms, S.D. (2008) PepA and ArgR do not regulate Cre recombination at the bacteriophage P1 loxP site. Plasmid, 59(2), pp. 119-126. (doi: 10.1016/j.plasmid.2007.12.001) (PMID:18226834) (PMCID:PMC2409434)
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Abstract
In the lysogenic state, bacteriophage P1 is maintained as a low copy-number circular plasmid. Site-specific recombination at <i>loxP</i> by the phage-encoded Cre protein keeps P1 monomeric, thus helping to ensure stable plasmid inheritance. Two <i>Escherichia</i> <i>coli</i> DNA-binding proteins, PepA and ArgR, were recently reported to be necessary for maintenance or establishment of P1 lysogeny. PepA and ArgR bind to regulatory DNA sequences upstream of the ColE1 <i>cer</i> recombination site to regulate site-specific recombination by the XerCD recombinases. This recombination keeps ColE1 in a monomeric state and helps to ensure stable plasmid maintenance. It has been suggested that ArgR and PepA play a similar role in P1 maintenance, regulating Cre recombination by binding to DNA sequences upstream of <i>loxP</i>. Here, we show that ArgR does not bind to its proposed binding site upstream of <i>loxP</i>, and that Cre recombination at <i>loxP</i> in its natural P1 context is not affected by PepA and ArgR in vitro. When sequences upstream of <i>loxP</i> were mutated to allow ArgR binding, PepA and ArgR still had no effect on Cre recombination. Our results demonstrate that PepA requires specific DNA sequences for binding, and that PepA and ArgR have no direct role in Cre recombination at P1 <i>loxP</i>.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | MacDonald, Dr Alasdair and Kilbride, Mrs Elizabeth and Colloms, Dr Sean |
| Authors: | MacDonald, A.I., Lu, Y., Kilbride, E.A., Akopian, A., and Colloms, S.D. |
| Subjects: | Q Science > QR Microbiology > QR355 Virology |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Plasmid |
| Publisher: | Elsevier Inc. |
| ISSN: | 0147-619X |
| ISSN (Online): | 1095-9890 |
| Published Online: | 28 January 2008 |
| Copyright Holders: | Copyright © 2007 Elsevier Inc. |
| First Published: | First published in Plasmid 59(2):119-126 |
| Publisher Policy: | Reproduced under a creative commons license |
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