dos Reis, F. C.G., Smith, B. O., Santos, C. C., Costa, T., F., Scharfstein, J., Coombs, G. H., Mottram, J. C. and Lima, A. P. C.A. (2008) The role of conserved residues of chagasin in the inhibition of cysteine peptidases. FEBS Letters, 582(4), pp. 485-490. (doi: 10.1016/j.febslet.2008.01.008)
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Publisher's URL: http://dx.doi.org/10.1016/j.febslet.2008.01.008
Abstract
We have evaluated the roles of key amino acids to the action of the natural inhibitor chagasin of papain-family cysteine peptidases. A W93A substitution decreased inhibitor affinity for human cathepsin L 100-fold, while substitutions of T31 resulted in 10-100-fold increases in the Ki for cruzipain of Trypanosoma cruzi. A T31A/T32A double mutant had increased affinity for cathepsin L but not for cruzipain, while the T31-T32 deletion drastically affected inhibition of both human and parasite peptidases. These differential effects reflect the occurrence of direct interactions between chagasin and helix 8 of cathepsin L, interactions that do not occur with cruzipain
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Mottram, Professor Jeremy and Smith, Dr Brian |
Authors: | dos Reis, F. C.G., Smith, B. O., Santos, C. C., Costa, T., F., Scharfstein, J., Coombs, G. H., Mottram, J. C., and Lima, A. P. C.A. |
Subjects: | Q Science > QH Natural history > QH345 Biochemistry |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | FEBS Letters |
Publisher: | Elsevier BV |
ISSN: | 0014-5793 |
ISSN (Online): | 1873-3468 |
Published Online: | 15 January 2008 |
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