The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography

Dalby, M.J. , Gadegaard, N. and Wilkinson, C.D.W. (2008) The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography. Journal of Biomedical Materials Research Part A, 84A(4), pp. 973-979. (doi:10.1002/jbm.a.31409)

Dalby, M.J. , Gadegaard, N. and Wilkinson, C.D.W. (2008) The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography. Journal of Biomedical Materials Research Part A, 84A(4), pp. 973-979. (doi:10.1002/jbm.a.31409)

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Publisher's URL: http://dx.doi.org/10.1002/jbm.a.31409

Abstract

It has been known for many years that cells will react to the shape of their microenvironment. It is more recently becoming clear that cells can alter their morphology, adhesions, and cytoskeleton in response to their nanoenvironment. A few studies have gone further and measured cellular response to high-adhesion nano-materials. There have, however, been practical difficulties associated with genomic studies focusing on low-adhesion nanotopographies. Because of advancement in fabrication techniques allowing the production of large area of structure and the ability to amplify mRNA prior to microarray hybridization, these difficulties can be overcome. Here, electron beam lithography has been used to fabricate arrays of pits with 120 nm diameters, 100 nm depth and 300 nm center to center spacing in hexagonal arrangement. Electron and fluorescent microscopies have been used to observe morphological changes in fibroblasts cultured on the pits. 1.7k gene microarray was used to gauge genomic response to the pits. The results show reduction in cellular adhesion, decrease in spreading, and a broad genomic down-regulation. Also noted was an increase in endocytotic activity in cells on the pits.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilkinson, Professor Christopher and Gadegaard, Professor Nikolaj and Dalby, Professor Matthew
Authors: Dalby, M.J., Gadegaard, N., and Wilkinson, C.D.W.
Subjects:R Medicine > R Medicine (General)
T Technology > T Technology (General)
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Biomedical Materials Research Part A
ISSN:1549-3296

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