Transcriptional responses of murine macrophages to the adenylate cyclase toxin of bordetella pertussis

Cheung, G.Y.C., Dickinson, P., Sing, G., Craigon, M., Ghazal, P., Parton, R. and Coote, J.G. (2008) Transcriptional responses of murine macrophages to the adenylate cyclase toxin of bordetella pertussis. Microbial Pathogenesis, 44(1), pp. 61-70. (doi: 10.1016/j.micpath.2007.08.007)

Abstract

Three different recombinant forms of CyaA were used to investigate transcriptional responses of murine bone marrow-derived macrophages (BMMs) using Affymetrix Mouse Genome GeneChips((R)). These forms were enzymically active, invasive CyaA, non-enzymically active, invasive CyaA (CyaA*) and non-enzymically active, non-invasive CyaA (proCyaA*). BMMs, treated with 20ng/ml of CyaA for 24h, showed over 1000 significant changes in gene transcription compared with control cells. CyaA caused an increase in transcription of many inflammatory genes and genes associated with various signalling cascades such as those involved in cyclic AMP-dependent protein kinase A signalling. Most strikingly, CyaA caused down-regulation of numerous genes involved in cell proliferation. CyaA* at 20ng/ml significantly up-regulated the transcription of only twelve genes after 24h whereas proCyaA* at this concentration significantly increased the transcription of only two genes.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Coote, Professor John and Parton, Dr Roger
• Authors: Cheung, G.Y.C., Dickinson, P., Sing, G., Craigon, M., Ghazal, P., Parton, R., and Coote, J.G.
• Subjects: Q Science > QR Microbiology; R Medicine > RB Pathology
• College/School: College of Medical Veterinary and Life Sciences; College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Microbial Pathogenesis
• ISSN: 0882-4010