The role of streptococcal plasmin(ogen) binding in infective endocarditis

Teles, C., Smith, A. , Ramage, G. and Lang, S. (2011) The role of streptococcal plasmin(ogen) binding in infective endocarditis. European Journal of Clinical Microbiology and Infectious Diseases, 30(1), pp. 127-129. (doi:10.1007/s10096-010-1053-5)

Teles, C., Smith, A. , Ramage, G. and Lang, S. (2011) The role of streptococcal plasmin(ogen) binding in infective endocarditis. European Journal of Clinical Microbiology and Infectious Diseases, 30(1), pp. 127-129. (doi:10.1007/s10096-010-1053-5)

Full text not currently available from Enlighten.

Abstract

Two phenotypic and three molecular methods were assessed for their ability to identify viridans group streptococci (VGS) to the species level. A panel of 23 clinical isolates, comprising strains isolated from infective endocarditis, blood cultures, pleural and peritoneal fluid, and 19 type/reference strains were analyzed. Identification was performed using two conventional phenotypic methods: API® rapid ID 32 Strep and the VITEK® 2 system, and genotypic analysis of the nucleotide sequence of the housekeeping gene sodA, restriction patterns generated by restriction fragment length polymorphism (RFLP) of the 16S rRNA gene and multilocus sequence analysis (MLSA) of seven housekeeping genes. The API® rapid ID 32 Strep accurately speciated 79% of the strains assessed, while the VITEK® 2 generated a successful identification for 55%, presenting limitations particularly with regard to species belonging to the mitis group. RFLP of the 16S rRNA gene correctly speciated 24% of the strains, having failed to allocate a species for 36% of the isolates examined. In contrast, sequence analysis of the sodA gene provided a correct identification for 95% of the strains assessed, while identification using the MLSA technique was unsuccessful due to practical limitations. The results generated herein indicate that no single methodology can be used to provide an accurate identification to the species level of all VGS, although nucleotide sequence analysis of the sodA gene proved to be useful in providing reliable speciation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon and Smith, Professor Andrew
Authors: Teles, C., Smith, A., Ramage, G., and Lang, S.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:European Journal of Clinical Microbiology and Infectious Diseases
ISSN:0934-9723
Published Online:11 September 2010

University Staff: Request a correction | Enlighten Editors: Update this record