Abatacept limits breach of self-tolerance in a Murine model of arthritis via effects on the generation of T follicular helper cells

Platt, A. M., Gibson, V. B., Patakas, A., Benson, R. A., Nadler, S. G., Brewer, J. M. , McInnes, I. and Garside, P. (2010) Abatacept limits breach of self-tolerance in a Murine model of arthritis via effects on the generation of T follicular helper cells. Journal of Immunology, 185(3), pp. 1558-1567. (doi:10.4049/jimmunol.1001311) (PMID:20601593)

Platt, A. M., Gibson, V. B., Patakas, A., Benson, R. A., Nadler, S. G., Brewer, J. M. , McInnes, I. and Garside, P. (2010) Abatacept limits breach of self-tolerance in a Murine model of arthritis via effects on the generation of T follicular helper cells. Journal of Immunology, 185(3), pp. 1558-1567. (doi:10.4049/jimmunol.1001311) (PMID:20601593)

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Abstract

Abatacept modulates CD28-mediated T cell costimulation and is efficacious in the treatment of rheumatoid arthritis (RA). Its mechanism of action has not been fully elucidated but will likely reveal critical pathologic pathways in RA. We show that abatacept substantially modulated Ag-specific T and B cell responses in vivo. Ag-specific T cell proliferation was reduced, and the acquisition of an activated phenotype, characterized by upregulation of CD69, OX40, ICOS, and programmed death-1 and downregulation of CD62L, was suppressed. Furthermore, abatacept suppressed the production of inflammatory cytokines, such as IFN-{gamma} and IL-17. These effects were associated with a failure of Ag-specific T cells to acquire the CXCR5+ICOS+ T follicular helper cell phenotype. This, in turn, led to a failure of these cells to enter B cell follicles, resulting in reduced specific Ab responses, despite normal B cell clonal expansion. To test the pathologic significance of this, we used a novel model of RA associated with breach of self-tolerance to self-Ag and demonstrated that abatacept prevented the emergence of self-reactivity. Thus, CD28-dependent signaling is required for optimal T follicular helper cell maturation and expansion, and its inhibition prevents loss of self-tolerance in a model of articular pathology. Thus, we provide a novel mode of action for abatacept with profound implications for its potential usefulness in early inflammatory arthropathies associated with autoantibody expression.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McInnes, Professor Iain and Garside, Professor Paul and Brewer, Professor James and Platt, Dr Andrew and Gibson, Miss Vivienne and Patakas, Dr Agapitos and Benson, Dr Robert
Authors: Platt, A. M., Gibson, V. B., Patakas, A., Benson, R. A., Nadler, S. G., Brewer, J. M., McInnes, I., and Garside, P.
Subjects:Q Science > QR Microbiology > QR180 Immunology
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Immunology
Journal Abbr.:J. Immunol.
Publisher:American Association of Immunologists
ISSN:0022-1767
ISSN (Online):1550-6606
Published Online:02 July 2010

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
497541DPFS Resource Request (University of Glasgow)Iain McInnesMedical Research Council (MRC)G0801687Infection Immunity and Inflammation Life Sciences