Sandison, M. E., Cumming, S. A., Kolch, W. and Pitt, A. R. (2010) On-chip immunoprecipitation for protein purification. Lab on a Chip, 10(20), pp. 2805-2813. (doi: 10.1039/C005295G) (PMID:20714512)
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Publisher's URL: http://dx.doi.org/10.1039/C005295G
Abstract
Immunoprecipitation (IP) is one of the most widely used and selective techniques for protein purification. Here, a miniaturised, polymer-supported immunoprecipitation ([small micro]IP) method for the on-chip purification of proteins from complex mixtures is described. A 4 [small micro]l PDMS column functionalised with covalently bound antibodies was created and all critical aspects of the [small micro]IP protocol (antibody immobilisation, blocking of potential non-specific adsorption sites, sample incubation and washing conditions) were assessed and optimised. The optimised [small micro]IP method was used to obtain purified fractions of affinity-tagged protein from a bacterial lysate
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Kolch, Prof Walter and Sandison, Dr Mairi and Pitt, Dr Andrew and Cumming, Dr Sarah |
| Authors: | Sandison, M. E., Cumming, S. A., Kolch, W., and Pitt, A. R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Lab on a Chip |
| Publisher: | Royal Society of Chemistry |
| ISSN: | 1473-0197 |
| ISSN (Online): | 1473-0189 |
| Published Online: | 17 August 2010 |
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