Orchestrating serine resolvases

Rice, P.A., Mouw, K.W., Montaño, S.P., Boocock, M.R., Rowland, S.-J. and Stark, W.M. (2010) Orchestrating serine resolvases. Biochemical Society Transactions, 38(2), pp. 384-387. (doi: 10.1042/BST0380384)

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Publisher's URL: http://dx.doi.org/10.1042/BST0380384

Abstract

A remarkable feature of the serine resolvases is their regulation: the wild-type enzymes will catalyse intra- but not inter-molecular recombination, can sense the relative orientation of their sites and can exchange strands directionally, despite the fact that there is no net release of chemical bond energy. The key to this regulation is that they are only active within a large intertwined complex called the 'synaptosome'. Because substrate topology greatly facilitates (or, in other cases, inhibits) formation of the synaptosome, it acts as a 'topological filter'. Within the defined topology of the synaptosome, strand exchange releases supercoiling tension, providing an energy source to bias the reaction direction. The regulatory portion of this complex contains additional copies of the recombinase and sometimes other DNA-bending proteins. We are using a combination of X-ray crystallography, biochemistry and genetics to model the full synaptic complex and to understand how the regulatory portion activates the crossover-site-bound recombinases

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Rowland, Dr Sally-Jane and Boocock, Dr Martin and Stark, Professor Marshall
Authors: Rice, P.A., Mouw, K.W., Montaño, S.P., Boocock, M.R., Rowland, S.-J., and Stark, W.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biochemical Society Transactions
ISSN:0300-5127

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