Barbaresi, S., Cortese, M. S., Quinn, J., Ashrafi, G. H., Graham, S.V. and Campo, M.S. (2010) Effects of human papillomavirus type 16 E5 deletion mutants on epithelial morphology: functional characterization of each transmembrane domain. Journal of General Virology, 91(2), pp. 521-530. (doi: 10.1099/vir.0.016295-0)
Full text not currently available from Enlighten.
Publisher's URL: http://dx.doi.org/10.1099/vir.0.016295-0
Abstract
Human papillomavirus type 16 (HPV-16) is the cause of cervical cancer. The HPV genome encodes three transforming proteins, E5, E6 and E7. E6 and E7 are the main transforming proteins of HPV while the role of E5 is still poorly understood. Using three dimensional organotypic raft cultures we show that HaCaT human keratinocytes expressing HPV-16 E5 form a very perturbed epithelium, with simultaneous hyperkeratinisation of some cells and defective differentiation of other cells. The basal layer is disturbed and many cells invade the collagen matrix. Many cells among the differentiated layers show characteristics of basal cells: progression through the cell cycle, expression of cytokeratin 14, lack of cytokeratin 1 and production of matrix metalloproteases (MMP). Using deletion mutants which encompass the three hydrophobic domains of E5, we have assigned the ability to invade the matrix to the first hydrophobic domain, and the capacity to induce MMP9 to the C-terminal four amino acids. We also show that invasion and production of MMP9 can be dissociated, as mutants still capable of invasion do not produce MMP9 and vice versa
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Graham, Professor Sheila |
Authors: | Barbaresi, S., Cortese, M. S., Quinn, J., Ashrafi, G. H., Graham, S.V., and Campo, M.S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Journal of General Virology |
Publisher: | Society for General Microbiology |
ISSN: | 0022-1317 |
ISSN (Online): | 1465-2099 |
University Staff: Request a correction | Enlighten Editors: Update this record