Preparation of Caco-2 cell sheets using plasma polymerised acrylic acid as a weak boundary layer

Majani, R., Zelzer, M., Gadegaard, N. , Rose, F.R. and Alexander, M.R. (2010) Preparation of Caco-2 cell sheets using plasma polymerised acrylic acid as a weak boundary layer. Biomaterials, 31(26), pp. 6764-6771. (doi:10.1016/j.biomaterials.2010.05.049)

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The use of cell sheets for tissue engineering applications has considerable advantages over single cell seeding techniques. So far, only thermoresponsive surfaces have been used to manufacture cell sheets without chemically disrupting the cell-surface interactions. Here, we present a new and facile technique to prepare sheets of epithelial cells using plasma polymerised acrylic acid films. The cell sheets are harvested by gentle agitation of the media without the need of any additional external stimulus. We demonstrate that the plasma polymer deposition conditions affect the viability and metabolic activity of the cells in the sheet and relate these effects to the different surface properties of the plasma polymerised acrylic acid films. Based on surface analysis data, a first attempt is made to explain the mechanism behind the cell sheet formation. The advantage of the epithelial cell sheets generated here over single cell suspensions to seed a PLGA scaffold is presented. The scaffold itself, prepared using a mould fabricated via photolithography, exhibits a unique architecture that mimics closely the dimensions of the native tissue (mouse intestine).

Item Type:Articles
Keywords:Adhesion Cell viability cells COATINGS CULTURE DEPOSITS electron beam lithography engineering films GRAFTS Intestine MEMBRANES plasma Plasma polymerisation Poly(lactic-co-glycolic acid) (PLGA) polymer RECONSTRUCTION STIMULUS SURFACE Surface analysis TEMPERATURE Tissue engineering TISSUE ENGINEERING APPLICATIONS
Glasgow Author(s) Enlighten ID:Gadegaard, Professor Nikolaj
Authors: Majani, R., Zelzer, M., Gadegaard, N., Rose, F.R., and Alexander, M.R.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biomaterials

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