Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis

Simon, G.C. , Schonteich, E. , Wu, C.C. , Piekny, A. , Ekiert, D. , Yu, X. , Gould, G.W. , Glotzer, M. and Prekeris, R. (2008) Sequential Cyk-4 binding to ECT2 and FIP3 regulates cleavage furrow ingression and abscission during cytokinesis. EMBO Journal, 27(13), pp. 1791-1803. (doi: 10.1038/emboj.2008.112) (PMID:18511905) (PMCID:PMC2486418)

Full text not currently available from Enlighten.

Abstract

Cytokinesis is a highly regulated and dynamic event that involves the reorganization of the cytoskeleton and membrane compartments. Recently, FIP3 has been implicated in targeting of recycling endosomes to the mid-body of dividing cells and is found required for abscission. Here, we demonstrate that the centralspindlin component Cyk-4 is a FIP3-binding protein. Furthermore, we show that FIP3 binds to Cyk-4 at late telophase and that centralspindlin may be required for FIP3 recruitment to the mid-body. We have mapped the FIP3-binding region on Cyk-4 and show that it overlaps with the ECT2-binding domain. Finally, we demonstrate that FIP3 and ECT2 form mutually exclusive complexes with Cyk-4 and that dissociation of ECT2 from the mid-body at late telophase may be required for the recruitment of FIP3 and recycling endosomes to the cleavage furrow. Thus, we propose that centralspindlin complex not only regulates acto-myosin ring contraction but also endocytic vesicle transport to the cleavage furrow and it does so through sequential interactions with ECT2 and FIP3.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gould, Professor Gwyn
Authors: Simon, G.C., Schonteich, E., Wu, C.C., Piekny, A., Ekiert, D., Yu, X., Gould, G.W., Glotzer, M., and Prekeris, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:EMBO Journal
ISSN:0261-4189
ISSN (Online):1460-2075
Published Online:29 May 2008

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
364581Functional analysis of the adipocyte secreted proteomeGwyn GouldBiotechnology and Biological Sciences Research Council (BBSRC)BBS/B/08825Institute of Molecular Cell and Systems Biology
403161The role of Rab 11-FIP3 and Rab 11-FIP4 in cytokinesisGwyn GouldBiotechnology and Biological Sciences Research Council (BBSRC)BB/D000017/1Institute of Molecular Cell and Systems Biology