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Measurement of urinary total 13C and 13C urea by isotope ratio mass spectrometry after administration of lactose [13C]-Ureide

Morrison, D., Dodson, B., and Preston, T. (1999) Measurement of urinary total 13C and 13C urea by isotope ratio mass spectrometry after administration of lactose [13C]-Ureide. Rapid Communications in Mass Spectrometry, 13 (13). pp. 1252-1256. ISSN 0951-4198 (doi:10.1002/(SICI)1097-0231(19990715)13:13<1252::AID-RCM565>3.0.CO;2-J)

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Abstract

A method of measuring total 13C excreted in urine after oral administration of lactose [13C]-ureide was developed using isotope ratio mass spectrometry. Furthermore, a method to measure 13C urea excreted in the urine was developed. Each urine sample collected over a 24 hour period, after administration of the tracer dose, was analysed for both total 13C and 13C urea. Combustion of the dried urine samples allowed measurement of the total 13C content. Treatment of urine samples with urease (EC 3.5.1.5) and analysis by isotope ratio mass spectrometry of the CO2 evolved allowed measurement of 13C urea in the urine sample. The total 13C and 13C urea contents of each urine sample, obtained throughout the protocol, were compared to total 13C and 13C urea contents of a urine sample taken before the test. This allowed calculation of the fraction of tracer incorporated into urea and the fraction of tracer excreted in total. Analyses showed that approximately 15% of the dose administered, in terms of 13C, was recovered in the urine over the sampling period. Further analysis for urinary 13C urea showed that less than 1% of the label was incorporated into urea excreted over the sampling period.

Item Type:Article
Status:Published
Refereed:Yes
Glasgow Author(s):Preston, Prof Thomas and Morrison, Dr Douglas
Authors: Morrison, D., Dodson, B., and Preston, T.
Subjects:G Geography. Anthropology. Recreation > GE Environmental Sciences
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Rapid Communications in Mass Spectrometry
ISSN:0951-4198
Published Online:15 July 1999

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