Measurement of urinary total 13C and 13C urea by isotope ratio mass spectrometry after administration of lactose [13C]-Ureide

Morrison, D. , Dodson, B. and Preston, T. (1999) Measurement of urinary total 13C and 13C urea by isotope ratio mass spectrometry after administration of lactose [13C]-Ureide. Rapid Communications in Mass Spectrometry, 13(13), pp. 1252-1256. (doi:10.1002/(SICI)1097-0231(19990715)13:13<1252::AID-RCM565>3.0.CO;2-J)

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Abstract

A method of measuring total <sup>13</sup>C excreted in urine after oral administration of lactose [<sup>13</sup>C]-ureide was developed using isotope ratio mass spectrometry. Furthermore, a method to measure <sup>13</sup>C urea excreted in the urine was developed. Each urine sample collected over a 24 hour period, after administration of the tracer dose, was analysed for both total <sup>13</sup>C and <sup>13</sup>C urea. Combustion of the dried urine samples allowed measurement of the total <sup>13</sup>C content. Treatment of urine samples with urease (EC 3.5.1.5) and analysis by isotope ratio mass spectrometry of the CO<sub>2</sub> evolved allowed measurement of <sup>13</sup>C urea in the urine sample. The total <sup>13</sup>C and <sup>13</sup>C urea contents of each urine sample, obtained throughout the protocol, were compared to total <sup>13</sup>C and <sup>13</sup>C urea contents of a urine sample taken before the test. This allowed calculation of the fraction of tracer incorporated into urea and the fraction of tracer excreted in total. Analyses showed that approximately 15% of the dose administered, in terms of <sup>13</sup>C, was recovered in the urine over the sampling period. Further analysis for urinary <sup>13</sup>C urea showed that less than 1% of the label was incorporated into urea excreted over the sampling period.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Preston, Professor Thomas and Morrison, Dr Douglas
Authors: Morrison, D., Dodson, B., and Preston, T.
Subjects:G Geography. Anthropology. Recreation > GE Environmental Sciences
College/School:College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Rapid Communications in Mass Spectrometry
ISSN:0951-4198
Published Online:15 July 1999

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