Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test

Shepherd, A.J., Williams, C.L., Doherty, C.P., Hossack, M., Preston, T. , McColl, K.E. and Weaver, L.T. (2001) Comparison of an enzyme immunoassay for the detection of Helicobacter pylori antigens in the faeces with the urea breath test. Archives of Disease in Childhood, 83, pp. 268-270.

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Abstract

<p>BACKGROUND: Current diagnostic tests for Helicobacter pylori are invasive (endoscopy) or indirect (urea breath test, serology). </p> <p>AIMS: To evaluate a new enzyme immunoassay (EIA) which detects H pylori antigens in faeces, by comparing its sensitivity and specificity in children with the (13)C urea breath test (UBT). </p> <p>METHODS: A total of 119 children underwent a UBT and provided a faecal sample for antigen testing within seven days. After an overnight fast each child provided a pretest breath sample, and samples at 30 and 40 minutes after ingestion of 100 mg (13)C labelled urea. (13)C enrichment of breath was measured by isotope ratio mass spectrometry. Faeces were stored at -70 degrees C until antigen testing, using the EIA. Samples were read spectrophotometrically at 450 nm and results were interpreted using recommended cut offs of optical density <0.14 as negative, >/=0.16 as positive, with >/=0.14 and <0. 16 representing equivocal results. Sensitivity and specificity were calculated using the manufacturer's cut off compared with UBT. </p> <p>RESULTS: Sensitivity and specificity were 88% and 82%, respectively. Negative and positive predictive values were 97% and 58%. </p> <p>CONCLUSIONS: The EIA is an alternative, non-invasive, and easy to use method for the detection of H pylori in children. Its high negative predictive value suggests a role in screening out uninfected children.</p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Weaver, Professor Lawrence and Preston, Professor Thomas and McColl, Mr Kenneth
Authors: Shepherd, A.J., Williams, C.L., Doherty, C.P., Hossack, M., Preston, T., McColl, K.E., and Weaver, L.T.
Subjects:Q Science > QR Microbiology > QR180 Immunology
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
College of Science and Engineering > Scottish Universities Environmental Research Centre
Journal Name:Archives of Disease in Childhood
Journal Abbr.:Arch. Dis. Child.
Publisher:B M J Group
ISSN:0003-9888
ISSN (Online):1468-2044
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