A fluorescence-based assay for the uptake of CPD0801 (DB829) by African trypanosomes

Ward, C. P., Burgess, K. E.V., Burchmore, R.J.S. , Barrett, M.P. and De Koning, H.P. (2010) A fluorescence-based assay for the uptake of CPD0801 (DB829) by African trypanosomes. Molecular and Biochemical Parasitology, 174(2), pp. 145-149. (doi:10.1016/j.molbiopara.2010.07.002)

Full text not currently available from Enlighten.

Publisher's URL: http://dx.doi.org/10.1016/j.molbiopara.2010.07.002

Abstract

Drug therapies currently used for second stage Human African Trypanosomiasis (HAT) exhibit problems with toxicity, difficulty of administration, and resistance linked to the loss of transporter function. Key to the development of new drugs for HAT is a better understanding of the transport properties of candidate compounds. Standard methods for studying transport utilize radio-labelled permeant or HPLC-MS, however the natural fluorescence of many trypanocidal compounds can be exploited. Here we present a fluorescence-based assay for measuring uptake, by trypanosomes, of CPD0801, a drug candidate for second stage HAT. Sample fluorescence is measured in a 96-well format using a benchtop fluorimeter. Our method is directly applicable to the study of other diamidines with similar fluorescent properties and readily adapted for use with other cell types or fluorescent molecules as we demonstrate for the veterinary trypanocide ethidium

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Burchmore, Dr Richard and De Koning, Professor Harry and Barrett, Professor Michael
Authors: Ward, C. P., Burgess, K. E.V., Burchmore, R.J.S., Barrett, M.P., and De Koning, H.P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Journal Abbr.:Mol. Biochem. Parasitol.
Publisher:Elsevier BV
ISSN:0166-6851
ISSN (Online):1872-9428

University Staff: Request a correction | Enlighten Editors: Update this record