Breast cancer patients' clinical outcome measures are associated with Src kinase family member expression

Elsberger, B., Fullerton, R., Zino, S., Jordan, F., Mitchell, T.J., Brunton, V.G., Mallon, E.A., Shiels, P.G. and Edwards, J. (2010) Breast cancer patients' clinical outcome measures are associated with Src kinase family member expression. British Journal of Cancer, 103(6), pp. 899-909. (doi:10.1038/sj.bjc.6605829)

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Abstract

<p>BACKGROUND: This study determined mRNA expression levels for Src kinase family (SFK) members in breast tissue specimens and assessed protein expression levels of prominent SFK members in invasive breast cancer to establish associations with clinical outcome. Ki67 was investigated to determine association between SFK members and proliferation.</p> <p>METHODS: The mRNA expression levels were assessed for eight SFK members by quantitative real-time PCR. Immunohistochemistry was performed for c-Src, Lyn, Lck and Ki67.</p> <p>RESULTS: mRNA expression was quantified in all tissue samples. SRC and LYN were the most highly expressed in malignant tissue. LCK was more highly expressed in oestrogen receptor (ER)-negative, compared with ER-positive tumours. High cytoplasmic Src kinase protein expression was significantly associated with decreased disease-specific survival. Lyn was not associated with survival at any cellular location. High membrane Lck expression was significantly associated with improved survival. Ki67 expression correlated with tumour grade and nuclear c-Src, but was not associated with survival.</p> <p>CONCLUSIONS: All eight SFK members were expressed in different breast tissues. Src kinase was highest expressed in breast cancer and had a negative impact on disease-specific survival. Membrane expression of Lck was associated with improved clinical outcome. High expression of Src kinase correlated with high proliferation.</p>

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Elsberger, Miss Beatrix and Shiels, Professor Paul and Edwards, Dr Joanne and Zino, Mr Samer
Authors: Elsberger, B., Fullerton, R., Zino, S., Jordan, F., Mitchell, T.J., Brunton, V.G., Mallon, E.A., Shiels, P.G., and Edwards, J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:British Journal of Cancer
Publisher:Nature Publishing Group
ISSN:0007-0920
ISSN (Online):1532-1827
Published Online:17 August 2010

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