Dalby, M.J. , Riehle, M.O. , Johnstone, H.J.H., Affrossman, S. and Curtis, A.S.G. (2003) Nonadhesive nanotopography: fibroblast response to poly(n-butyl methacrylate)-poly(styrene) demixed surface features. Journal of Biomedical Materials Research Part A, 67A(3), pp. 1025-1032. (doi: 10.1002/jbm.a.10139)
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Publisher's URL: http://dx.doi.org/10.1002/jbm.a.10139
Abstract
It is becoming clear that cells do not only respond to micrometric scale topography, but may also respond to topography at the nanometric scale. Nano-fabrication methods such as electron beam lithography are, however, expensive and time consuming. Polymer demixing of poly(styrene) and poly(4-bromostyrene) has been found to produce nano-scale islands of reproducible height, and the islands have been previously shown to effect cell events such as adhesion, spreading, proliferation, and differentiation. This study uses demixed poly(styrene) and poly(<i>n</i>-butyl methacrylate) to produce nano-islands with closer packing and narrower widths compared with those previously studied. Observations have been made of morphological and cytoskeletal changes in human fibroblasts interacting with 10- and 50-nm-high islands. The methods used included scanning electron microscopy, fluorescent microscopy, and optical microscopy. The results indicated that the cells do not respond differently to the 10-nm islands compared with planar samples but, in contrast, the 50-nm islands are nonadhesive.
Item Type: | Articles |
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Keywords: | Nanobiotechnology, topography, fibroblasts, cytoskeleton, adhesion. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Curtis, Professor Adam and Dalby, Professor Matthew and Riehle, Dr Mathis |
Authors: | Dalby, M.J., Riehle, M.O., Johnstone, H.J.H., Affrossman, S., and Curtis, A.S.G. |
Subjects: | R Medicine > R Medicine (General) T Technology > TP Chemical technology |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Journal of Biomedical Materials Research Part A |
ISSN: | 1549-3296 |
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