Cell response to dextran-derivatised iron oxide nanoparticles post internalisation

Berry, C.C. , Wells, S., Charles, S., Aitchison, G. and Curtis, A.S.G. (2004) Cell response to dextran-derivatised iron oxide nanoparticles post internalisation. Biomaterials, 25(23), pp. 5405-5413. (doi:10.1016/j.biomaterials.2003.12.046)

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Publisher's URL: http://dx.doi.org/10.1016/j.biomaterials.2003.12.046

Abstract

Magnetic nanoparticles have been used for many years as magnetic resonance imaging contrast agents. Despite the fact that there are currently several dextran-coated iron oxide nanoparticles are in preclinical and clinical use, there is very little information available concerning the influence such particles have on cells in culture. The prerequisite for particles employed as contrast agents is capture and subsequent uptake by cells. This study involved the use of magnetic nanoparticles synthesised and derivatised with dextran, compared to similar underivatised plain particles. The influence in vitro was assessed using human dermal fibroblasts and various techniques to observe cell–particles interaction, including light and fluorescence microscopy, scanning and transmission electron microscopy. The results indicate that although both the uncoated and the dextran-derivatised particles are uptaken into the cell, the derivatised particles induce alterations in cell behaviour and morphology distinct from the plain particles, suggesting that cell response is dependent on the particles coating.

Item Type:Articles
Keywords:Nanoparticle, magnetism, dextran, in vitro test, cell viability, cell culture, fluorescence.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Curtis, Professor Adam and Berry, Dr Catherine
Authors: Berry, C.C., Wells, S., Charles, S., Aitchison, G., and Curtis, A.S.G.
Subjects:T Technology > TP Chemical technology
College/School:College of Medical Veterinary and Life Sciences
University Centres > Glasgow Materials Research Initiative
Journal Name:Biomaterials
ISSN:0142-9612

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