p53 activates ICAM-1 (CD54) expression in an NF-kappaB-independent manner

Gorgoulis, V.G., Zacharatos, P., Kotsinas, A., Kletsas, D., Mariatos, G., Zoumpourlis, V., Ryan, K.M. , Kittas, C. and Papavassiliou, A.G. (2003) p53 activates ICAM-1 (CD54) expression in an NF-kappaB-independent manner. EMBO Journal, 22(7), pp. 1567-1578. (doi:10.1093/emboj/cdg157)

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Publisher's URL: http://dx.doi.org/10.1093/emboj/cdg157

Abstract

Intercellular adhesion molecule-1 (ICAM-1) is a crucial receptor in the cell-cell interaction, a process central to the reaction to all forms of injury. Its expression is upregulated in response to a variety of inflammatory/immune mediators, including cellular stresses. The NF-kappaB signalling pathway is known to be important for activation of ICAM-1 transcription. Here we demonstrate that ICAM-1 induction represents a new cellular response to p53 activation and that NF-kappaB inhibition does not prevent the effect of p53 on ICAM-1 expression after DNA damage. Induction of ICAM-1 is abolished after treatment with the specific p53 inhibitor pifithrin-alpha and is abrogated in p53-deficient cell lines. Furthermore, we map two functional p53- responsive elements to the introns of the ICAM-1 gene, and show that they confer inducibility to p53 in a fashion similar to other p53 target genes. These results support an NF-kappaB-independent role for p53 in ICAM-1 regulation that may link p53 to ICAM-1 function in various physiological and pathological settings.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ryan, Professor Kevin
Authors: Gorgoulis, V.G., Zacharatos, P., Kotsinas, A., Kletsas, D., Mariatos, G., Zoumpourlis, V., Ryan, K.M., Kittas, C., and Papavassiliou, A.G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:EMBO Journal
ISSN:0261-4189

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