Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase

Kelly, E.E., Horgan, C.P., Adams, C., Patzer, T.M., Ní Shúilleabháin, D.M., Norman, J.C. and McCaffrey, M.W. (2009) Class I Rab11-family interacting proteins are binding targets for the Rab14 GTPase. Biology of the Cell, 102(1), pp. 51-62. (doi: 10.1042/BC20090068)

Abstract

Background information. Rab11 and Rab14 are two related Rab GTPases that are believed to function in endosomal recycling and Golgi/endosome transport processes. We, and others, have identified a group of proteins that interact with Rab11 and function as Rab11 effectors, known as the Rab11-FIPs (family interacting proteins). This protein family has been sub-classified into two groups - class I FIPs [FIP2, RCP (Rab coupling protein) and Rip11 (Rab11 interacting protein)] and class II FIPs (FIP3 and FIP4). Results. In the present study we identify the class I FIPs as dual Rab-binding proteins by demonstrating that they also interact with Rab14 in a GTP-dependent manner. We show that these interactions are specific for the class I FIPs and that they occur via their C-terminal regions, which encompass the previously described RBD (Rab11 binding domain). Furthermore, we show that Rab14 significantly co-localizes with the TfnR (transferrin receptor) and that Rab14 Q70L co-localizes with Rab11 a and with the class I FIPs on the ERC (endosomal recycling compartment) during interphase. Additionally, we show that during cytokinesis Rab14 localizes to the cleavage furrow/midbody. Conclusions. The data presented in the present study, which identifies the class I FIPs as the first putative effector proteins for the Rab14 GTPase, indicates greater complexity in the Rab-binding specificity of the class I FIP proteins.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Norman, Professor James and Adams, Miss Christine
• Authors: Kelly, E.E., Horgan, C.P., Adams, C., Patzer, T.M., Ní Shúilleabháin, D.M., Norman, J.C., and McCaffrey, M.W.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Biology of the Cell
• ISSN: 0248-4900