Investigation of the molecular nature of low-molecular-mass cobalt ions in isolated osteoarthritic knee-joint synovial fluid

Silwood, C.J.L., Chikanza, I.C., Tanner, K.E. , Shelton, J.C., Bowsher, J.G. and Grootveld, M. (2004) Investigation of the molecular nature of low-molecular-mass cobalt ions in isolated osteoarthritic knee-joint synovial fluid. Free Radical Research, 38(6), pp. 562-571. (doi: 10.1080/10715760410001684630)

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Publisher's URL: http://dx.doi.org/10.1080/10715760410001684630

Abstract

Abstract High field 1H NMR spectroscopy demonstrated that addition of Co(II) ions to osteoarthritic knee-joint synovial fluid (SF) resulted in its complexation by a range of biomolecules, the relative efficacies of these complexants/chelators being citrate >> histidine threonine>>glycine glutamate glutamine phenylalanine tyrosine > formate > lactate>>alanine > valine > acetate > pyruvate > creatinine, this order reflecting the ability of these ligands to compete for the available Co(II) in terms of (1) thermodynamic equilibrium constants for the formation of their complexes and (2) their SF concentrations. Since many of these SF Co(II) complexants (e.g. histidinate) serve as powerful •OH scavengers, the results acquired indicate that any of this radical generated from the Co(II) source in such complexes via Fenton or pseudo-Fenton reaction systems will be "site-specifically" scavenged. The significance of these observations with regard to cobalt toxicity and the in vivo corrosion of cobalt-containing metal alloy joint prostheses (e.g. CoCr alloys) is discussed.

Item Type:Articles
Keywords:Low-molecular-mass cobalt ions; Osteoarthritis; 1H NMR; Speciation; Synovial fluid; CoCr metal alloy joint prostheses
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Tanner, Professor Kathleen
Authors: Silwood, C.J.L., Chikanza, I.C., Tanner, K.E., Shelton, J.C., Bowsher, J.G., and Grootveld, M.
Subjects:R Medicine > R Medicine (General)
Q Science > QD Chemistry
T Technology > T Technology (General)
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Free Radical Research
ISSN:1071-5762

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