Quince, C., Lanzen, A., Curtis, T.P., Davenport, R.J., Hall, N., Head, I.M., Read, F., and Sloan, W.T. (2009) Accurate determination of microbial diversity from 454 pyrosequencing data. Nature Methods, 6 (9). 639-U27. ISSN 1548-7091 (doi:10.1038/NMETH.1361 )
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We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.
|Glasgow Author(s):||Quince, Dr Christopher and Sloan, Prof William and Curtis, Prof Thomas|
|Authors:||Quince, C., Lanzen, A., Curtis, T.P., Davenport, R.J., Hall, N., Head, I.M., Read, F., and Sloan, W.T.|
|Subjects:||T Technology > TA Engineering (General). Civil engineering (General)|
|College/School:||College of Science and Engineering > School of Engineering > Infrastructure and Environment|
|Journal Name:||Nature Methods|