Quince, C., Lanzen, A., Curtis, T.P., Davenport, R.J., Hall, N., Head, I.M., Read, F. and Sloan, W.T. (2009) Accurate determination of microbial diversity from 454 pyrosequencing data. Nature Methods, 6(9), 639-U27. (doi: 10.1038/NMETH.1361) (PMID:19668203)
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Abstract
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Sloan, Professor William and Quince, Dr Christopher |
| Authors: | Quince, C., Lanzen, A., Curtis, T.P., Davenport, R.J., Hall, N., Head, I.M., Read, F., and Sloan, W.T. |
| Subjects: | T Technology > TA Engineering (General). Civil engineering (General) |
| College/School: | College of Science and Engineering > School of Engineering > Infrastructure and Environment |
| Journal Name: | Nature Methods |
| ISSN: | 1548-7091 |
| ISSN (Online): | 1548-7105 |
| Published Online: | 09 August 2009 |
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