Antimicrobial activity of Coniothyrium minitans and its macrolide antibiotic macrosphelide A

Tomprefa, N., McQuilken, M. P., Hill, R. A. and Whipps, J. M. (2009) Antimicrobial activity of Coniothyrium minitans and its macrolide antibiotic macrosphelide A. Journal of Applied Microbiology, 106(6), pp. 2048-2056. (doi: 10.1111/j.1365-2672.2009.04174.x)

Full text not currently available from Enlighten.

Abstract

Aims: Assessment of antimicrobial activity of the mycoparasite Coniothyrium minitans and its macrolide antibiotic macrosphelide A. Methods and Results: Thirteen isolates of C minitans were tested for ability to inhibit a number of filamentous fungi, yeasts, oomycetes and bacteria in agar based tests. Activity was found against some ascomycetes, basidiomycetes, oomycetes and Gram-positive bacteria, but not against zygomycetes, yeasts or Gram-negative bacteria tested. Six C. minitans isolates (Conio, Contans, IVT1, CM/AP/3118, B279/1, Al/327/1) were found to produce macrosphelide A in liquid culture and no other antibiotics were detected. On agar, macrosphelide A inhibited growth of some ascomycetes, basidiomycetes, oomycetes and all four Gram-positive bacteria tested, including the medically important Staphylococcus aureus with a minimum inhibitory concentration of <= 500 mu g ml(-1). There was no inhibition observed against the yeasts and Gram-negative bacteria when macrosphelide A was tested at 700 mu g ml(-1). Conclusions: The spectrum and level of activity of macrosphelide A produced by C. minitans against micro-organisms are extended markedly compared to previous reports. Significance and Impact of the Study: Macrosphelide A was effective against Staph. aureus. Further study on the control of this bacterium is merited in view of the development of antibiotic resistance.

Item Type:Articles
Keywords:APOTHECIAL PRODUCTION BIOCONTROL Coniothyrium minitans DISEASE FUNGI GROWTH LETTUCE macrosphelide A minimum inhibitory concentration RATES RESISTANCE SCLEROTINIA-SCLEROTIORUM SPECTRA SUBSTANCES
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hill, Professor Robert
Authors: Tomprefa, N., McQuilken, M. P., Hill, R. A., and Whipps, J. M.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Journal of Applied Microbiology
ISSN:1364-5072

University Staff: Request a correction | Enlighten Editors: Update this record