Poly(L-proline) II Helix Propensities in Poly(L-lysine) Dendrigraft Generations from Vibrational Raman Optical Activity

Johannessen, C., Kapitan, J., Collet, H., Commeyras, A., Hecht, L. and Barron, L.D. (2009) Poly(L-proline) II Helix Propensities in Poly(L-lysine) Dendrigraft Generations from Vibrational Raman Optical Activity. Biomacromolecules, 10(6), pp. 1662-1664. (doi: 10.1021/bm9002249)

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Abstract

Vibrational Raman optical activity (ROA), measured as small circularly polarized components in Raman scattering from chiral molecules, was applied to study the backbone conformations of the first five generations Of Poly(L-lysine) dendrigrafts (DGLs) in water. Generation I was found to support predominantly the Poly(L-proline) If (PPII) conformation, the amount of which steadily decreased with increasing generation, with a concomitant increase in other backbone conformations. This behavior may be due to increasing crowding of the lysine side chains, together with suppression of backbone hydration, with increasing branching. In contrast, the ROA spectra of a series of linear poly(L-lysine)s in water show little change with increasing molecular weight. Our results may have implications for the nonimmunogenic properties of DGLs.

Item Type:Articles
Keywords:BEHAVIOR Chiral CHIRAL MOLECULES COMPONENTS CONFORMATION HYPERBRANCHED POLYMERS INTRINSIC DISORDER MOLECULE MOLECULES OPTICAL-ACTIVITY PEPTIDES POLYPEPTIDES POLYPROLINE-II Raman optical activity RAMAN-SCATTERING RECOGNITION SCATTERING SPECTRA UNFOLDED PROTEINS WATER
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kapitan, Dr Josef and Hecht, Dr Lutz and Barron, Professor Laurence and Johannessen, Dr Christian
Authors: Johannessen, C., Kapitan, J., Collet, H., Commeyras, A., Hecht, L., and Barron, L.D.
College/School:College of Science and Engineering > School of Chemistry
Journal Name:Biomacromolecules
ISSN:1525-7797

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