In Situ Detection of Cell Wall Polysaccharides in Sitka Spruce (Picea Sitchensis (Bong.) Carriere) Wood Tissue

Altaner, C., Knox, J. P. and Jarvis, M. C. (2007) In Situ Detection of Cell Wall Polysaccharides in Sitka Spruce (Picea Sitchensis (Bong.) Carriere) Wood Tissue. Bioresources, 2(2), pp. 284-295.

Abstract

Wood cell wall polysaccharides can be probed with monoclonal antibodies and carbohydrate-binding modules (CBMs). Binding of monoclonal antibodies to beta-1-4-xylan, beta-1-4-mannan, beta-1-3-glucan, and beta-1-5-arabinan structures were observed in native Sitka spruce (Picea sitchensis (Bong.) Carriere) wood cell walls. Furthermore CBMs of different families, differing in their affinities for crystalline cellulose (3a) and amorphous cellulose (17 and 28), were shown to bind to the native wood cell walls with varying intensities. Resin channel forming cells exhibited an increased beta-1-4-xylan and a decreased beta-1-4-mannan content. Focusing on severe compression wood (CW) tracheids, beta-1-3glucan was found towards the cell lumen. In contrast, beta-1-5-arabinan structures were present in the intercellular spaces between the round tracheids in severe CW, highlighting the importance of this polymer in cell adhesion.

• Item Type: Articles
• Keywords: Alpha-1-5-Arabinan, Arabinogalactan-Proteins, Beta-1-3-Glucan, Beta-1-4-Mannan, Beta-1-4-Xylan, Binding, Carbohydrate-Binding Module (Cbm), Carbohydrate-Binding Modules, Cell, Cells, Cellulose, Compression Wood, Crystalline Cellulose, Family, Immunofluorescence, In-Situ, Kraft Pulp, Localization, Monoclonal Antibodies, Monoclonal Antibody, Monoclonal-Antibodies, Pinus-Radiata, Polysaccharide, Polysaccharides, Pulp Fibers, Sitka Spruce, Sitka Spruce (Picea Sitchensis (Bong.) Carriere), Spruce, Structure, Tension Wood, Tracheid, Tracheids, Wood
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Jarvis, Dr Michael and Altaner, Dr Clemens
• Authors: Altaner, C., Knox, J. P., and Jarvis, M. C.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Bioresources
• ISSN: 1930-2126