Felmlee, D. J. et al. (2010) Intravascular transfer contributes to postprandial increase in numbers of very-low-density hepatitis C virus particles. Gastroenterology, 139(5), pp. 1774-1783. (doi: 10.1053/j.gastro.2010.07.047) (PMID:20682323)
Full text not currently available from Enlighten.
Abstract
<b>Background and Aims</b>: The physical association of hepatitis C virus (HCV) particles with lipoproteins in plasma results in distribution of HCV in a broad range of buoyant densities. This association is thought to increase virion infectivity by mediating cell entry via lipoprotein receptors. We sought to determine if factors that affect triglyceride-rich lipoprotein (TRL) metabolism alter the density and dynamics of HCV particles in the plasma of patients with chronic HCV infection. <b>Methods</b>: Fasting patients (n=10) consumed a high-fat milkshake; plasma was collected and fractionated by density gradients. HCV RNA was measured in the very-low–density fraction (VLDF, d<1.025 g/mL) before and at 7 serial time points postprandially. <b>Results</b>: The amount of HCV RNA in the VLDF (HCVVLDF) increased a mean of 26-fold, peaking 180 minutes after the meal (P<0.01). Quantification of HCV RNA throughout the density gradient fractions revealed that HCVVLDF rapidly disappeared, rather than migrating into the adjacent density fraction. Immuno-affinity separation of the VLDF, using antibodies that recognize apoB-100 and not apoB-48 showed that HCVVLDF is composed of chylomicron- and VLDL-associated HCV particles; peaking 120 and 180 minutes after the meal, respectively. Plasma from fasting HCV-infected patients mixed with uninfected plasma increased the quantity of HCVVLDF, compared to that mixed with phosphate-buffered saline, demonstrating extracellular assembly of HCVVLDF. <b>Conclusions</b>: Dietary triglyceride alters the density and dynamics of HCV in plasma. The rapid clearance rate of HCVVLDF indicates that association with TRL is important for HCV infectivity. HCV particles, like exchangeable apolipoproteins, appear to reassociate with TRLs in the vascular compartment.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Caslake, Professor Muriel and Packard, Professor Chris and McLauchlan, Professor John |
| Authors: | Felmlee, D. J., Sheridan, D. A., Bridge, S. H., Nielsen, S. U., Milne, R. W., Packard, C. J., Caslake, M. J., McLauchlan, J., Toms, G. L., Neely, R. D. G., and Bassendine, M. F. |
| College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Gastroenterology |
| Publisher: | Elsevier B.V. |
| ISSN: | 0016-5085 |
| ISSN (Online): | 1528-0012 |
| Published Online: | 02 August 2010 |
University Staff: Request a correction | Enlighten Editors: Update this record
Altmetric
Altmetric