Fraser, N.L.W., Rowley, G., Field, M. and Stott, D.I. (2003) The VH gene repertoire of splenic B cells and somatic hypermutation in systemic lupus erythematosus. Arthritis Research and Therapy, 5, pp. 114-121. (doi: 10.1186/ar627)
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Abstract
In systemic lupus erythematosus (SLE) it has been hypothesized that self-reactive B cells arise from virgin B cells that express low-affinity, nonpathogenic germline V genes that are cross-reactive for self and microbial antigens, which convert to high-affinity autoantibodies via somatic hypermutation. The aim of the present study was to determine whether the V<sub>H</sub> family repertoire and pattern of somatic hypermutation in germinal centre (GC) B cells deviates from normal in SLE. Rearranged immunoglobulin V<sub>H</sub> genes were cloned and sequenced from GCs of a SLE patient's spleen. From these data the GC V gene repertoire and the pattern of somatic mutation during the proliferation of B-cell clones were determined. The results highlighted a bias in V<sub>H</sub>5 gene family usage, previously unreported in SLE, and under-representation of the V<sub>H</sub>1 family, which is expressed in 20–30% of IgM+ B cells of healthy adults and confirmed a defect in negative selection. This is the first study of the splenic GC response in human SLE.
Item Type: | Articles |
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Keywords: | Spleen, systematic lupus erythematosus. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Stott I, Professor David |
Authors: | Fraser, N.L.W., Rowley, G., Field, M., and Stott, D.I. |
Subjects: | Q Science > QR Microbiology > QR180 Immunology |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Arthritis Research and Therapy |
Publisher: | BioMed Central |
ISSN: | 1478-6354 |
ISSN (Online): | 1478-6362 |
Copyright Holders: | Copyright © 2003 BioMed Central |
First Published: | First published in Arthritis Research and Therapy 5:114-121 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher. |
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