Spectral trends in the fluorescence of single bacterial light-harvesting complexes: experiments and modified redfield simulations

Rutkauskas, D., Novoderezhkin, V., Gall, A., Olsen, J., Cogdell, R.J. , Hunter, C.N. and van Grondelle, R. (2006) Spectral trends in the fluorescence of single bacterial light-harvesting complexes: experiments and modified redfield simulations. Biophysical Journal, 90(7), pp. 2475-2485. (doi:10.1529/biophysj.105.075903)

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Abstract

In this work we present and discuss the single-molecule fluorescence spectra of a variety of species of light-harvesting complexes: LH2 of Rhodopseudomonas acidophila, Rhodobacter sphaeroides, and Rhodospirillum molischianum and LH1 of Rhodobacter sphaeroides. The emission spectrum of these complexes varies as a function of time as was described in earlier work. For each type of complex, we observe a pronounced and well-reproducible characteristic relationship between the fluorescence spectral parameters of the peak wavelength, width, and asymmetry. This dependence for the LH2 complexes can be quantitatively explained on the basis of a disordered exciton model by varying the static disorder and phonon coupling parameters. In addition, a correlation of the pigment site energies has to be assumed to interpret the behavior of the LH1 complex.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard
Authors: Rutkauskas, D., Novoderezhkin, V., Gall, A., Olsen, J., Cogdell, R.J., Hunter, C.N., and van Grondelle, R.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biophysical Journal

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