Comparative study of spectral flexibilities of bacterial light-harvesting complexes: structural implications

Rutkauskas, D., Olsen, J., Gall, A., Cogdell, R.J. , Hunter, C.N. and van Grondelle, R. (2006) Comparative study of spectral flexibilities of bacterial light-harvesting complexes: structural implications. Biophysical Journal, 90(7), pp. 2463-2474. (doi:10.1529%2Fbiophysj.105.075895)

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Abstract

This work presents a comparative study of the frequencies of spectral jumping of individual light-harvesting complexes of six different types: LH2 of Rhodopseudomonas acidophila, Rhodobacter sphaeroides, and Rhodospirillum molischianum; LH1 of Rhodobacter sphaeroides; and two “domain swap mutants” of LH2 of Rhodobacter sphaeroides: PACLH1 and PACLH2mol, in which the α-polypeptide C-terminus is exchanged with the corresponding sequence from LH1 of Rhodobacter sphaeroides or LH2 of Rhodospirillum molischianum, respectively. The quasistable states of fluorescence peak wavelength that were previously observed for the LH2 of Rps. acidophila were confirmed for other species. We also observed occurrences of extremely blue-shifted spectra, which were associated with reversible bleaching of one of the chromophore rings. Different jumping behavior is observed for single complexes of different types investigated with the same equivalent excitation intensity. The differences in spectral diffusion are associated with subtle differences of the binding pocket of B850 pigments and the structural flexibility of the different types of complexes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cogdell, Professor Richard
Authors: Rutkauskas, D., Olsen, J., Gall, A., Cogdell, R.J., Hunter, C.N., and van Grondelle, R.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Biophysical Journal

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