Small interfering RNA targeting bovine papillomavirus type 1 E2 induces apoptosis in equine sarcoid transformed fibroblasts

Gobeil, P.A.M., Yuan, Z.Q., Gault, E.A., Morgan, I.M., Campo, M.S. and Nasir, L. (2009) Small interfering RNA targeting bovine papillomavirus type 1 E2 induces apoptosis in equine sarcoid transformed fibroblasts. Virus Research, 145(1), pp. 162-165. (doi: 10.1016/j.virusres.2009.06.019)

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Abstract

Equine sarcoids are skin tumours of horses caused by infection with BPV-1 or 2. Maintenance and replication of the viral genome depend upon the viral proteins E1 and E2. We examined the effects of an E2 specific siRNA on E2 and E1 viral gene expression, viral load and cell growth in BPV-1 transformed sarcoid-derived cells. Transfection with E2-siRNA caused a reduction in E2 and E1 mRNA expression as well as viral load, growth inhibition and decreased anchorage-independent growth. siRNA treated cells showed significantly higher apoptosis rates than control cells. Thus sequence specific targeting of E2 provides a powerful strategy to eliminate BPV-1 genomes and induce cell death in BPV-1 transformed cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Campo, M Saveria and Gault, Mrs Elizabeth and Gobeil, Mr Philipe and Nasir, Professor Lubna and Morgan, Professor Iain and Yuan, Dr Zhengqiang
Authors: Gobeil, P.A.M., Yuan, Z.Q., Gault, E.A., Morgan, I.M., Campo, M.S., and Nasir, L.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Virus Research
ISSN:0168-1702

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