C-reactive protein quantification in porcine saliva: A minimally invasive test for pig health monitoring

Gutierrez, A.M., Martinez-Subiela, S., Eckersall, P.D. and Ceron, J.J. (2009) C-reactive protein quantification in porcine saliva: A minimally invasive test for pig health monitoring. Veterinary Journal, 181(3), pp. 261-265. (doi:10.1016/j.tvjl.2008.03.021)

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Abstract

Study objectives were to investigate whether C-reactive protein (CRP) in pig saliva Could be quantified using an adapted, time-resolved immunofluorometry assay (TR-IFMA), and to determine whether the assay Could distinguish healthy front diseased animals. The test method bad intra- and inter-assay coefficients of variation of 5.75%, and 9.73%, respectively, the limit of detection was 0.47 ng/mL and the coefficient of determination was 0.98. Analysis of CRP concentrations in paired serum and saliva samples front 50 pigs gave it positive correlation (r = 0.702, P < 0.01) and the salivary CRP concentration was able to distinguish healthy front diseased animals ill 62 samples from pigs With naturally occurring or experimentally-induced inflammation. The results suggest that this minimally invasive. straightforward and sensitive assay may be useful in pig health and welfare monitoring.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor Peter
Authors: Gutierrez, A.M., Martinez-Subiela, S., Eckersall, P.D., and Ceron, J.J.
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Veterinary Journal
Publisher:Elsevier Ltd
ISSN:1090-0233
ISSN (Online):1532-2971

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