The human papillomavirus 16 E2 protein is stabilised in S phase

Johansson, C., Graham, S.V. , Dornan, E.S. and Morgan, I.M. (2009) The human papillomavirus 16 E2 protein is stabilised in S phase. Virology, 394(2), pp. 194-199. (doi:10.1016/j.virol.2009.08.046) (PMID:19781729)

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Abstract

The human papillomavirus 16 E2 protein regulates transcription from, and replication of, the viral genome and is also required for segregation of the viral genome via interaction with mitotic bodies. To regulate DNA replication E2 interacts with sequences around the origin of replication and recruits the viral helicase E1 via a protein-protein interaction, which then initiates viral genome replication. The replication role of E2 must originally function in a host cell S phase. In this report, we demonstrate that E2 is stabilised in the S phase of the cell cycle and that this stabilisation is accompanied by an increase in phosphorylation of the protein. This increased phosphorylation and stability are likely required for optimum viral DNA replication and therefore identification of the enzymes involved in regulating these properties of E2 will provide targets for therapeutic intervention in the viral life cycle. Preliminary studies have identified E2 as a Cdk2 substrate demonstrating this enzyme as a candidate kinase for mediating the in vivo phosphorylation of HPV16 E2.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Graham, Professor Sheila and Johansson, Dr Cecilia and Dornan, Mr Edward and Morgan, Professor Iain
Authors: Johansson, C., Graham, S.V., Dornan, E.S., and Morgan, I.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
Publisher:Elsevier
ISSN:0042-6822
ISSN (Online):1096-0341
Published Online:24 September 2009
Copyright Holders:Copyright © 2009 Elsevier Inc.
First Published:First published in Virology 394(2):194-199
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
396081A therapeutic strategy for improving treatment of virally induced gynaecological cancers and pre-maglignant tumoursIain MorganWellbeing of Women (WELLBEING)UNSPECIFIEDMVLS III - CENTRE FOR VIRUS RESEARCH