Human papillomavirus E1 and E2 mediated DNA replication is not arrested by DNA damage signalling

King, L.E., Fisk, J.C., Dornan, E.S., Donaldson, M.M. , Melendy, T. and Morgan, I.M. (2010) Human papillomavirus E1 and E2 mediated DNA replication is not arrested by DNA damage signalling. Virology, 406(1), pp. 95-102. (doi:10.1016/j.virol.2010.06.033)

King, L.E., Fisk, J.C., Dornan, E.S., Donaldson, M.M. , Melendy, T. and Morgan, I.M. (2010) Human papillomavirus E1 and E2 mediated DNA replication is not arrested by DNA damage signalling. Virology, 406(1), pp. 95-102. (doi:10.1016/j.virol.2010.06.033)

Full text not currently available from Enlighten.

Abstract

Integration of human papillomaviruses into that of the host promotes genomic instability and progression to cancer; factors that promote integration remain to be fully identified. DNA damage agents can promote double strand breaks during DNA replication providing substrates for integration and we investigated the ability of DNA damage to regulate HPV E1 and E2 mediated DNA replication. Results demonstrate that HPV E1 and E2 replication is not arrested following DNA damage, both in vivo and in vitro, while replication by SV40 Large T antigen is arrested and ATR is the candidate kinase for mediating the arrest. LTAg is a target for PIKK DNA damage signalling kinases, while E1 is not. We propose that the failure of E1 to be targeted by PIKKs allows HPV replication in the presence of DNA damaging agents. Such replication will result in double strand breaks in the viral genome ultimately promoting viral integration and cervical cancer

Item Type:Articles
Keywords:Dna veterinary
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dornan, Mr Edward and Morgan, Professor Iain and Donaldson, Dr Mary and Melendy, Dr Thomas
Authors: King, L.E., Fisk, J.C., Dornan, E.S., Donaldson, M.M., Melendy, T., and Morgan, I.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virology
ISSN:0042-6822
Published Online:31 July 2010

University Staff: Request a correction | Enlighten Editors: Update this record