A novel Src kinase inhibitor reduces tumour formation in a skin carcinogenesis model

Serrels, B. et al. (2009) A novel Src kinase inhibitor reduces tumour formation in a skin carcinogenesis model. Carcinogenesis, 30(2), pp. 249-257. (doi:10.1093/carcin/bgn278)

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Publisher's URL: http://dx.doi.org/10.1093/carcin/bgn278

Abstract

The Src family tyrosine kinases are key modulators of cancer cell invasion and metastasis and a number of Src kinase inhibitors are currently in clinical development for the treatment of solid tumours. However, there is growing evidence that Src is also upregulated at very early stages of epithelial cancer development. We have investigated the role of Src in mouse skin, which is one of the most tractable models of epithelial homoeostasis and tumorigenesis. We found that Src protein expression and activity was regulated during the normal hair cycle and was increased specifically during the proliferative anagen phase and also in response to the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). AZD0530, a selective Src inhibitor, prevented the TPA-induced proliferation of basal keratinocytes both in vivo and in vitro. Moreover, treatment with AZD0530 reduced papilloma formation following the well-established 7,12-dimethylbenz(a)anthracene/TPA skin carcinogenesis protocol but did not inhibit the subsequent proliferation of the papillomas. Furthermore, AZD0530 did not alter the malignant conversion of papillomas to squamous cell carcinoma suggesting a role for Src in early tumour development in the skin carcinogenesis model, rather than at later stages of tumour progression. Src expression and activity were also seen in human actinic keratoses that are hyperproliferative pre-malignant skin lesions, indicating that Src may also play a role in the early stages of human skin tumour development. Thus, Src inhibitors such as AZD0530 may therefore have chemopreventative properties in patients with hyperproliferative epidermal disorders

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ashton, Dr Gabrielle and Canel, Dr Marta and Serrels, Mr Alan and Baldeschi, Miss Christine and Doyle, Dr Brendan and Mason, Miss Susan and Sansom, Professor Owen and Serrels, Dr Bryan
Authors: Serrels, B., Serrels, A., Mason, S. M., Baldeschi, C., Ashton, G. H., Canel, M., Mackintosh, L. J., Doyle, B., Green, T. P., Frame, M. C., Sansom, O. J., and Brunton, V. G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Journal Name:Carcinogenesis
Publisher:Oxford University Press
ISSN:0143-3334
ISSN (Online):1460-2180
Published Online:05 December 2008
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
446751The role of human papillomaviruses and their oncoproteins in non melanoma skin cancerIain MorganMedical Research Council (MRC)G0601648Centre for Virus Research